Molecular cloning and over-expression of the glyoxylate bypass operon from Escherichia coli ML308

el-Mansi, E.M.T., MacKintosh, C., Duncan, K., Holms, W.H. and Nimmo, H.G. (1987) Molecular cloning and over-expression of the glyoxylate bypass operon from Escherichia coli ML308. Biochemical Journal, 242(3), pp. 661-665. (doi: 10.1042/bj2420661) (PMID:3297049) (PMCID:PMC1147762)

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Abstract

A recombinant plasmid carrying an 11 kb restriction-endonuclease-ClaI fragment of genomic DNA from Escherichia coli ML308 was constructed. This plasmid complements an aceA mutation. The plasmid encodes the structural genes of the glyoxylate bypass operon, namely malate synthase A (aceB), isocitrate lyase (aceA) and isocitrate dehydrogenase kinase/phosphatase (aceK), as judged by overexpression of enzyme activities and transcription/translation experiments in vitro. Subcloning confirmed that expression of the aceK gene is essential for growth on acetate.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Nimmo, Professor Hugh
Authors: el-Mansi, E.M.T., MacKintosh, C., Duncan, K., Holms, W.H., and Nimmo, H.G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Biochemical Journal
Publisher:Portland Press
ISSN:0264-6021
ISSN (Online):1470-8728

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