White, P.J., Nairn, J., Price, N.C., Nimmo, H.G. , Coggins, J.R. and Hunter, I.S. (1992) Phosphoglycerate mutase from Streptomyces coelicolor A3(2): Purification and characterization of the enzyme and cloning and sequence analysis of the gene. Journal of Bacteriology, 174(2), pp. 434-440. (doi: 10.1128/jb.174.2.434-440.1992) (PMID:1530847) (PMCID:PMC205734)
Full text not currently available from Enlighten.
Abstract
The enzyme 3-phosphoglycerate mutase was purified 192-fold from Streptomyces coelicolor, and its N-terminal sequence was determined. The enzyme is tetrameric with a subunit Mr of 29,000. It is 2,3-bisphosphoglycerate dependent and inhibited by vanadate. The gene encoding the enzyme was cloned by using a synthetic oligonucleotide probe designed from the N-terminal peptide sequence, and the complete coding sequence was determined. The deduced amino acid sequence is 64% identical to that of the phosphoglycerate mutase of Saccharomyces cerevisiae and has substantial identity to those of other phosphoglycerate mutases.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Nimmo, Professor Hugh and Price, Prof Nicholas and Coggins, Professor John |
| Authors: | White, P.J., Nairn, J., Price, N.C., Nimmo, H.G., Coggins, J.R., and Hunter, I.S. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Medical Veterinary and Life Sciences > School of Life Sciences |
| Journal Name: | Journal of Bacteriology |
| Publisher: | American Society for Microbiology |
| ISSN: | 0021-9193 |
| ISSN (Online): | 1098-5530 |
University Staff: Request a correction | Enlighten Editors: Update this record
Deposit and Record Details
Deposit and Record Details