Vascular bed-targeted in vivo gene delivery using tropism-modified adeno-associated viruses

Work, L.M. et al. (2006) Vascular bed-targeted in vivo gene delivery using tropism-modified adeno-associated viruses. Molecular Therapy, 13(4), pp. 683-693. (doi: 10.1016/j.ymthe.2005.11.013)

Abstract

Virus-mediated gene delivery is restricted by the infectivity profile of the chosen vector. Targeting the vascular endothelium via systemic delivery has been attempted using peptides isolated in vitro (using either phage or vector display) and implicit reliance on target receptor expression in vivo. This has limited application since endothelial cells in vitro and in vivo differ vastly in receptor profiles and because of the existence of complex endothelial "zip codes" in vivo. We therefore tested whether in vivo phage display combined with adeno-associated virus (AAV) capsid modifications would allow in vivo homing to the endothelium residing in defined organs. Extensive in vivo biopanning in rats identified four consensus peptides homing to the lung or brain. Each was incorporated into the VP3 region of the AAV-2 capsid to display the peptide at the virion surface. Peptides that conferred heparan independence were shown to retarget virus to the expected vascular bed in vivo in a preferential manner, determined 28 days post-systemic injection by both virion DNA and transgene expression profiling. Our findings significantly impact the design of viral vectors for targeting individual vascular beds in vivo.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Nicklin, Professor Stuart and Baker, Professor Andrew and Denby, Dr Laura and Work, Dr Lorraine and Britton, Ms Nicola
• Authors: Work, L.M., Buning, H., Hunt, E., Nicklin, S.A., Denby, L., Britton, N., Leike, K., Odenthal, M., Drebber, U., Hallek, M., and Baker, A.H.
• College/School: College of Medical Veterinary and Life Sciences; College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
• Journal Name: Molecular Therapy
• Publisher: Nature Publishing Group
• ISSN: 1525-0016
• ISSN (Online): 1525-0024
• Published Online: 01 January 2006

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
32937	1	Development and evaluation of novel adeno-associated viral vectors for long term gene therapy	Andrew Baker	Medical Research Council (MRC)	G0200279	Institute of Cardiovascular and Medical Sciences