McCulloch, K., Huesa, C., Dunning, L., Litherland, G. J., Van 'T Hof, R. J., Lockhart, J. C. and Goodyear, C. S. (2019) Accelerated post traumatic osteoarthritis in a dual injury murine model. Osteoarthritis and Cartilage, 27(12), pp. 1800-1810. (doi: 10.1016/j.joca.2019.05.027) (PMID:31283983)
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Abstract
Objective: Joint injury involving destabilisation of the joint and damage to the articular cartilage (e.g., sports-related injury) can result in accelerated post-traumatic osteoarthritis (PTOA). Destabilised medial meniscotibial ligament (DMM) surgery is one of the most commonly used murine models and whilst it recapitulates Osteoarthritis (OA) pathology, it does not necessarily result in multi-tissue injury, as occurs in PTOA. We hypothesised that simultaneous cartilage damage and joint destabilisation would accelerate the onset of OA pathology. Methods: OA was induced in C57BL/6 mice via (a) DMM, (b) microblade scratches of articular cartilage (CS) or (c) combined DMM and cartilage scratch (DCS). Mice were culled 7, 14 and 28 days post-surgery. Microcomputed tomography (μCT) and histology were used to monitor bone changes and inflammation. Dynamic weight bearing, an indirect measure of pain, was assessed on day 14. Results: Osteophytogenesis analysis via μCT revealed that osteophytes were present in all groups at days 7 and 14 post-surgery. However, in DCS, osteophytes were visually larger and more numerous when compared with DMM and cartilage scratch (CS). Histological assessment of cartilage at day 14 and 28, revealed significantly greater damage in DCS compared with DMM and CS. Furthermore, a significant increase in synovitis was observed in DCS. Finally, at day 14 osteophyte numbers correlated with changes in dynamic weight bearing. Conclusion: Joint destabilisation when combined with simultaneous cartilage injury accelerates joint deterioration, as seen in PTOA. Thus, DCS provides a novel and robust model for investigating multiple pathological hallmarks, including osteophytogenesis, cartilage damage, synovitis and OA-related pain.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was supported by a Programme Grant from Versus Arthritis (Grant #20199) and a PhD studentship from the University of West of Scotland. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Dunning, Mrs Lynette and McCulloch, Kendal and Goodyear, Professor Carl and Lockhart, Professor John and Huesa, Dr Carmen and Litherland, Dr Gary |
| Authors: | McCulloch, K., Huesa, C., Dunning, L., Litherland, G. J., Van 'T Hof, R. J., Lockhart, J. C., and Goodyear, C. S. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing |
| Research Centre: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Immunobiology |
| Journal Name: | Osteoarthritis and Cartilage |
| Publisher: | Elsevier |
| ISSN: | 1063-4584 |
| ISSN (Online): | 1522-9653 |
| Published Online: | 05 July 2019 |
| Copyright Holders: | Copyright © 2019 The Authors |
| First Published: | First published in Osteoarthritis and Cartilage 27(12):1800-1810 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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