Traveling surface acoustic wave (TSAW) microfluidic fluorescence activated cell sorter (μFACS)

Mutafopulos, K., Spink, P., Lofstrom, C.D., Lu, P.J., Lu, H., Sharpe, J.C., Franke, T. and Weitz, D.A. (2019) Traveling surface acoustic wave (TSAW) microfluidic fluorescence activated cell sorter (μFACS). Lab on a Chip, 19(14), pp. 2435-2443. (doi: 10.1039/C9LC00163H) (PMID:31192328)

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Abstract

We report a microfluidic fluorescence activated cell-sorting (μFACS) device that employs traveling surface acoustic waves (TSAW) to sort cells at rates comparable to conventional jet-in-air FACS machines, with high purity and viability. The device combines inertial flow focusing and sheath flow to align and evenly space cells, improving the sorting accuracy and screening rate. We sort with an interdigital transducer (IDT) whose tapered geometry allows precise positioning of the TSAW for optimal cell sorting. We sort three different cell lines at several kHz, at cell velocities exceeding one meter per second, while maintaining both sorting purity and cell viability at around 90% simultaneously.

Item Type:Articles
Additional Information:This work was supported by the Harvard Materials Research Science and Engineering Center, National Science Foundation DMR-1420570, and Cytonome/ST, LLC (Bedford, Massachusetts). This work was performed in part at the Center for Nanoscale Systems (CNS), a member of the National Nanotechnology Coordinated Infrastructure Network (NNCI), which is supported by the National Science Foundation under NSF award no. 1541959.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Franke, Professor Thomas
Authors: Mutafopulos, K., Spink, P., Lofstrom, C.D., Lu, P.J., Lu, H., Sharpe, J.C., Franke, T., and Weitz, D.A.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Lab on a Chip
Publisher:Royal Society of Chemistry
ISSN:1473-0197
ISSN (Online):1473-0189
Published Online:13 June 2019

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