bcl-x(L) and RAG genes are induced and the response to IL-2 enhanced in E mu EBNA-1 transgenic mouse lymphocytes

Tsimbouri, P. , Drotar, M.E., Coy, J.L. and Wilson, J.B. (2002) bcl-x(L) and RAG genes are induced and the response to IL-2 enhanced in E mu EBNA-1 transgenic mouse lymphocytes. Oncogene, 21, pp. 5182-5187. (doi:10.1038/sj.onc.1205490)

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Abstract

We have described transgenic mice expressing Epstein-Barr virus (EBV) nuclear antigen-1 (EBNA-1) in B-cells which show a predisposition to lymphoma. To investigate the underlying oncogenic mechanisms, we have cross bred transgenic strains of mice, examined the pre-tumour B-cell phenotype and investigated the expression levels of selected cellular genes as a response to EBNA-1 expression. We have found that bcl-xL and the recombination activating genes (RAG) 1 and 2 are induced in pre-neoplastic samples of EBNA-1 expressing mice. Induction of bcl-xL may explain the observed redundancy in lymphomagenesis between transgenic EBNA-1 and bcl-2. In addition, bone marrow cells derived from the EEBNA-1 mice show a greater capacity for cultured growth compared to controls, particularly in the presence of IL-2. Notably, bcl-xL expression is responsive to IL-2. These data shed new light on the potential contribution of EBNA-1 to EBV associated tumorigenicity as well as to the viral life cycle and open a potential avenue for therapeutic intervention.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Wilson, Professor Joanna and Tsimbouri, Dr Monica and Drotar, Mr Mark
Authors: Tsimbouri, P., Drotar, M.E., Coy, J.L., and Wilson, J.B.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Oncogene
ISSN:0950-9232

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