Double-Cubic Point Spread Function for 3D Extended-Depth Localization Microscopy

Zhou, Y. , Zickus, V., Harvey, A. R. and Zammit, P. (2018) Double-Cubic Point Spread Function for 3D Extended-Depth Localization Microscopy. In: Imaging and Applied Optics 2018, Orlando, FL, USA, 25-28 Jun 2018, CM3E.4. ISBN 9781943580446 (doi:10.1364/COSI.2018.CM3E.4)

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Abstract

We report the design and implementation of a new pupil-engineered phase function that enables simple and robust 3D localization microscopy with a ten-fold extension in depth-of-field. Applications include single-particle tracking, super resolution microscopy and lab-on-chip. We demonstrate its application to in vivo mapping of blood flow in zebrafish.

Item Type:Conference Proceedings
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Zhou, Mr Yongzhuang and Zammit, Mr Paul and Harvey, Professor Andy
Authors: Zhou, Y., Zickus, V., Harvey, A. R., and Zammit, P.
College/School:College of Science and Engineering > School of Physics and Astronomy
ISBN:9781943580446

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