Causes and effects of loss of classical non-homologous end joining pathway in parasitic eukaryotes

Nenarokova, A., Záhonová, K., Krasilnikova, M., Gahura, O., McCulloch, R. , Zíková, A., Yurchenko, V. and Lukeš, J. (2019) Causes and effects of loss of classical non-homologous end joining pathway in parasitic eukaryotes. mBio, 10(4), e01541-9. (doi: 10.1128/mBio.01541-19) (PMID:31311886) (PMCID:PMC6635534)

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Abstract

We report frequent losses of components of the classical nonhomologous end joining pathway (C-NHEJ), one of the main eukaryotic tools for end joining repair of DNA double-strand breaks, in several lineages of parasitic protists. Moreover, we have identified a single lineage among trypanosomatid flagellates that has lost Ku70 and Ku80, the core C-NHEJ components, and accumulated numerous insertions in many protein-coding genes. We propose a correlation between these two phenomena and discuss the possible impact of the C-NHEJ loss on genome evolution and transition to the parasitic lifestyle.

Item Type:Articles
Additional Information:Support from the Czech Grant Agency 18-15962S and the ERD Funds, project OPVVV 16_019/0000759, to J.L. and V.Y., ERC CZ LL1601 to J.L., and the Czech Grant Agency 18-17529S to A.Z. is acknowledged. Work in R.M.’s lab is supported by the BBSRC (BB/K006495/1, BB/M028909/1, and BB/N016165/1) and the Wellcome Trust (206815 and 104111).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McCulloch, Professor Richard and Krasilnikova, Marija
Authors: Nenarokova, A., Záhonová, K., Krasilnikova, M., Gahura, O., McCulloch, R., Zíková, A., Yurchenko, V., and Lukeš, J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:mBio
Publisher:American Society for Microbiology
ISSN:2150-7511
ISSN (Online):2150-7511
Published Online:16 July 2019
Copyright Holders:Copyright © 2019 Nenarokova et al.
First Published:First published in mBio 10(4):e01541-19
Publisher Policy:Reproduced under a Creative Commons license

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
606431Kinase dependent control of DNA replication and repair as a drug target in Trypanosoma brucei.Richard McCullochBiotechnology and Biological Sciences Research Council (BBSRC)BB/K006495/1III - PARASITOLOGY
68706114CONFAP Understanding diverged genome repair and replication functions in trypanosomatid parasitesRichard McCullochBiotechnology and Biological Sciences Research Council (BBSRC)BB/M028909/1III - PARASITOLOGY
716221How do common and diverged features of the replicative stress response shape the biology of TriTrypRichard McCullochBiotechnology and Biological Sciences Research Council (BBSRC)BB/N016165/1III - PARASITOLOGY
716221How do common and diverged features of the replicative stress response shape the biology of TriTrypRichard McCullochBiotechnology and Biological Sciences Research Council (BBSRC)BB/N016165/1III - PARASITOLOGY
371799The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOTR)104111/Z/14/Z & AIII - PARASITOLOGY