Advances in 3D single particle localization microscopy

Zhou, Y. , Handley, M., Carles, G. and Harvey, A. R. (2019) Advances in 3D single particle localization microscopy. APL Photonics, 4(6), 060901. (doi: 10.1063/1.5093310)

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The spatial resolution of conventional optical microscopy is limited by diffraction to transverse and axial resolutions of about 250 nm, but localization of point sources, such as single molecules or fluorescent beads, can be achieved with a precision of 10 nm or better in each direction. Traditional approaches to localization microscopy in two dimensions enable high precision only for a thin in-focus layer that is typically much less than the depth of a cell. This precludes, for example, super-resolution microscopy of extended three-dimensional biological structures or mapping of blood velocity throughout a useful depth of vasculature. Several techniques have been reported recently for localization microscopy in three dimensions over an extended depth range. We describe the principles of operation and typical applications of the most promising 3D localization microscopy techniques and provide a comparison of the attainable precision for each technique in terms of the Cramér-Rao lower bound for high-resolution imaging.

Item Type:Articles
Additional Information:Also funded by the China Scholarship Council (Grant No. 201503170229).
Glasgow Author(s) Enlighten ID:Handley, Mr Michael and Carles Santacana, Dr Guillem and Zhou, Mr Yongzhuang and Harvey, Professor Andy
Authors: Zhou, Y., Handley, M., Carles, G., and Harvey, A. R.
College/School:College of Science and Engineering > School of Physics and Astronomy
Journal Name:APL Photonics
Publisher:AIP Publishing
ISSN (Online):2378-0967
Published Online:04 June 2019
Copyright Holders:Copyright © 2019 The Authors
First Published:First published in APL Photonics 4(6):060901
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
636381EPSRC Centre for Doctoral Training in Sensing and MeasurementAndrew HarveyEngineering and Physical Sciences Research Council (EPSRC)EP/L016753/1S&E P&A - PHYSICS & ASTRONOMY
729121Seeing within extended volumes and 3D structures: 3D imaging and applicationsGuillem Carles SantacanaLeverhulme Trust (LEVERHUL)ECF-2016-757S&E P&A - PHYSICS & ASTRONOMY