Wolbachia transinfections in Culex quinquefasciatus generate cytoplasmic incompatibility

Ant, T.H., Herd, C., Louis, F., Failloux, A.B. and Sinkins, S.P. (2019) Wolbachia transinfections in Culex quinquefasciatus generate cytoplasmic incompatibility. Insect Molecular Biology, (doi:10.1111/imb.12604) (PMID:31194893) (Early Online Publication)

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Abstract

Culex quinquefasciatus is an important mosquito vector of a number of viral and protozoan pathogens of humans and animals, and naturally carries the endosymbiont Wolbachia pipientis, strain wPip. Wolbachia are used in two distinct vector control strategies: firstly, population suppression caused by mating incompatibilities between mass‐released transinfected males and wild females; and secondly, the spread of pathogen transmission‐blocking strains through populations. Using embryonic microinjection, two novel Wolbachia transinfections were generated in C. quinquefasciatus using strains native to the mosquito Aedes albopictus: a wAlbB single infection, and a wPip plus wAlbA superinfection. The wAlbB infection showed full bidirectional cytoplasmic incompatibility (CI) with wild‐type C. quinquefasciatus in reciprocal crosses. The wPipwAlbA superinfection showed complete unidirectional CI, and therefore population invasion potential. Whereas the wAlbB strain showed comparatively low overall densities, similar to the native wPip, the wPipwAlbA superinfection reached over 400‐fold higher densities in the salivary glands compared to the native wPip, suggesting it may be a candidate for pathogen transmission blocking.

Item Type:Articles
Additional Information:This work was also supported by the Emerging and Major Infectious Diseases of Livestock (EMIDA) European Research Area Networks (ERA Net) (contract No 219235).
Status:Early Online Publication
Refereed:Yes
Glasgow Author(s) Enlighten ID:Sinkins, Professor Steven and Ant, Dr Thomas and Herd, Dr Christie
Authors: Ant, T.H., Herd, C., Louis, F., Failloux, A.B., and Sinkins, S.P.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Insect Molecular Biology
Publisher:Wiley
ISSN:0962-1075
ISSN (Online):1365-2583
Published Online:13 June 2019
Copyright Holders:Copyright © 2019 The Authors
First Published:First published in Insect Molecular Biology 2019
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
772321Wolbachia-based control of virus transmission by the mosquito Aedes albopictusSteven SinkinsWellcome Trust (WELLCOTR)108508/Z/15/ZMVLS III - CENTRE FOR VIRUS RESEARCH
732881Wolbachia-mediated arbovirus inhibition in mosquitoesSteven SinkinsWellcome Trust (WELLCOTR)202888/Z/16/ZMVLS III - CENTRE FOR VIRUS RESEARCH