Ant, T.H., Herd, C., Louis, F., Failloux, A.B. and Sinkins, S.P. (2020) Wolbachia transinfections in Culex quinquefasciatus generate cytoplasmic incompatibility. Insect Molecular Biology, 29(1), pp. 1-8. (doi: 10.1111/imb.12604) (PMID:31194893) (PMCID:PMC7027843)
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Abstract
Culex quinquefasciatus is an important mosquito vector of a number of viral and protozoan pathogens of humans and animals, and naturally carries the endosymbiont Wolbachia pipientis, strain wPip. Wolbachia are used in two distinct vector control strategies: firstly, population suppression caused by mating incompatibilities between mass‐released transinfected males and wild females; and secondly, the spread of pathogen transmission‐blocking strains through populations. Using embryonic microinjection, two novel Wolbachia transinfections were generated in C. quinquefasciatus using strains native to the mosquito Aedes albopictus: a wAlbB single infection, and a wPip plus wAlbA superinfection. The wAlbB infection showed full bidirectional cytoplasmic incompatibility (CI) with wild‐type C. quinquefasciatus in reciprocal crosses. The wPipwAlbA superinfection showed complete unidirectional CI, and therefore population invasion potential. Whereas the wAlbB strain showed comparatively low overall densities, similar to the native wPip, the wPipwAlbA superinfection reached over 400‐fold higher densities in the salivary glands compared to the native wPip, suggesting it may be a candidate for pathogen transmission blocking.
| Item Type: | Articles |
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| Additional Information: | This work was also supported by the Emerging and Major Infectious Diseases of Livestock (EMIDA) European Research Area Networks (ERA Net) (contract No 219235). |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Sinkins, Professor Steven and Ant, Dr Thomas and Herd, Dr Christie |
| Authors: | Ant, T.H., Herd, C., Louis, F., Failloux, A.B., and Sinkins, S.P. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Insect Molecular Biology |
| Publisher: | Wiley |
| ISSN: | 0962-1075 |
| ISSN (Online): | 1365-2583 |
| Published Online: | 13 June 2019 |
| Copyright Holders: | Copyright © 2019 The Authors |
| First Published: | First published in Insect Molecular Biology 29(1): 1-8 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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