The endoplasmic reticulum unfolded protein response varies depending on the affected region of the tissue but independently from the source of stress

Perochon, J. , Grandon, B., Roche, D., Wintz, C., Demay, Y., Mignotte, B., Szuplewski, S. and Gaumer, S. (2019) The endoplasmic reticulum unfolded protein response varies depending on the affected region of the tissue but independently from the source of stress. Cell Stress and Chaperones, 24(4), pp. 817-824. (doi: 10.1007/s12192-019-01009-8) (PMID:31144193) (PMCID:PMC6629755)

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Abstract

Accumulation of unfolded proteins and calcium dyshomeostasis induces endoplasmic reticulum (ER) stress, which can be resolved by the unfolded protein response (UPR). We have previously reported that activation of the PERK/ATF4 branch of the UPR, by overexpressing Presenilin in part of the vestigial domain of Drosophila wing imaginal discs, induces both a caspase-dependent apoptosis and a Slpr/JNK/Dilp8-dependent developmental delay that allows compensation of cell death in the tissue. Recently, dDad1 depletion in Drosophila in engrailed-expressing cells of wing imaginal discs was also reported to activate the PERK/ATF4 branch but induced Mekk1/JNK-dependent apoptosis. Here, we assessed whether the stressed cell location in the wing imaginal disc could explain these differences in response to chronic ER stress or whether the stress source could be responsible for the signaling discrepancy. To address this question, we overexpressed a Rhodopsin-1 mutant prone to aggregate either in vestigial- or engrailed-expressing cells. We observed similar responses to the Presenilin overexpression in the vestigial domain and to the dDad1 depletion in the engrailed domain. Therefore, the consequences of a PERK/ATF4 branch activation depend on the position of the cell in the Drosophila wing imaginal disc, suggesting interactions of PERK signaling with developmental pathways involved in the determination or maintenance of wing domains.

Item Type:Articles
Keywords:Apoptosis, homeostasis, PERK, UPR, wing imaginal disc.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Perochon, Miss Jessica
Authors: Perochon, J., Grandon, B., Roche, D., Wintz, C., Demay, Y., Mignotte, B., Szuplewski, S., and Gaumer, S.
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Journal Name:Cell Stress and Chaperones
Publisher:Springer
ISSN:1355-8145
ISSN (Online):1466-1268
Published Online:30 May 2019

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