Bacteria-laden microgels as autonomous three-dimensional environments for stem cell engineering

Witte, K. , Rodrigo-Navarro, A. and Salmeron-Sanchez, M. (2019) Bacteria-laden microgels as autonomous three-dimensional environments for stem cell engineering. Materials Today Bio, 2, 100011. (doi:10.1016/j.mtbio.2019.100011)

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Abstract

A one-step microfluidic system is developed in this study which enables the encapsulation of stem cells and genetically engineered non-pathogenic bacteria into a so-called three-dimensional (3D) pearl lace–like microgel of alginate with high level of monodispersity and cell viability. The alginate-based microgel constitutes living materials that control stem cell differentiation in either an autonomous or heteronomous manner. The bacteria (Lactococcus lactis) encapsulated within the construct surface display adhesion fragments (III7-10 fragment of human fibronectin) for integrin binding while secreting growth factors (recombinant human bone morphogenetic protein-2) to induce osteogenic differentiation of human bone marrow–derived mesenchymal stem cells. We concentrate on interlinked pearl lace microgels that enabled us to prototype a low-cost 3D bioprinting platform with highly tunable properties.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel and Mohammadi, Dr Kimia and Rodrigo-Navarro, Mr Aleixandre
Authors: Witte, K., Rodrigo-Navarro, A., and Salmeron-Sanchez, M.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Materials Today Bio
Publisher:Elsevier
ISSN:2590-0064
ISSN (Online):2590-0064
Published Online:18 June 2019
Copyright Holders:Copyright © 2019 The Authors
First Published:First published in Materials Today Bio 2:100011
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
722061Engineering growth factor microenvironments- a new therapeutic paradigm for regenerative medicineManuel Salmeron-SanchezEngineering and Physical Sciences Research Council (EPSRC)EP/P001114/1ENG - BIOMEDICAL ENGINEERING

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