Bacteria-laden microgels as autonomous three-dimensional environments for stem cell engineering

Witte, K., Rodrigo-Navarro, A. and Salmeron-Sanchez, M. (2019) Bacteria-laden microgels as autonomous three-dimensional environments for stem cell engineering. Materials Today Bio, 2, 100011. (doi: 10.1016/j.mtbio.2019.100011)

Abstract

A one-step microfluidic system is developed in this study which enables the encapsulation of stem cells and genetically engineered non-pathogenic bacteria into a so-called three-dimensional (3D) pearl lace–like microgel of alginate with high level of monodispersity and cell viability. The alginate-based microgel constitutes living materials that control stem cell differentiation in either an autonomous or heteronomous manner. The bacteria (Lactococcus lactis) encapsulated within the construct surface display adhesion fragments (III7-10 fragment of human fibronectin) for integrin binding while secreting growth factors (recombinant human bone morphogenetic protein-2) to induce osteogenic differentiation of human bone marrow–derived mesenchymal stem cells. We concentrate on interlinked pearl lace microgels that enabled us to prototype a low-cost 3D bioprinting platform with highly tunable properties.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Salmeron-Sanchez, Professor Manuel and Witte, Dr Kimia and Rodrigo-Navarro, Mr Aleixandre
• Authors: Witte, K., Rodrigo-Navarro, A., and Salmeron-Sanchez, M.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: Materials Today Bio
• Publisher: Elsevier
• ISSN: 2590-0064
• ISSN (Online): 2590-0064
• Published Online: 18 June 2019
• Copyright Holders: Copyright © 2019 The Authors
• First Published: First published in Materials Today Bio 2:100011
• Publisher Policy: Reproduced under a Creative Commons License

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
72206	1	Engineering growth factor microenvironments- a new therapeutic paradigm for regenerative medicine	Manuel Salmeron-Sanchez	Engineering and Physical Sciences Research Council (EPSRC)	EP/P001114/1	ENG - BIOMEDICAL ENGINEERING