Genetic variation and potential for genetic improvement of cuticle deposition on chicken eggs

Dunn, I. C., Woolliams, J. A., Wilson, P. W., Icken, W., Cavero, D., Jones, A. C., Quinlan-Pluck, F., Williams, G. O. S., Olori, V. and Bain, M. M. (2019) Genetic variation and potential for genetic improvement of cuticle deposition on chicken eggs. Genetics Selection Evolution, 51(1), 25. (doi:10.1186/s12711-019-0467-5) (PMID:31164080) (PMCID:PMC6549311)

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Abstract

Background: The cuticle is an invisible glycosylated protein layer that covers the outside of the eggshell and forms a barrier to the transmission of microorganisms. Cuticle-specific staining and in situ absorbance measurements have been used to quantify cuticle deposition in several pure breeds of chicken. For brown eggs, a pre-stain and a post-stain absorbance measurement is required to correct for intrinsic absorption by the natural pigment. For white eggs, a post-stain absorbance measurement alone is sufficient to estimate cuticle deposition. The objective of the research was to estimate genetic parameters and provide data to promote adoption of the technique to increase cuticle deposition and reduce vertical transmission of microorganisms. Results: For all pure breeds examined here, i.e. Rhode Island Red, two White Leghorns, White Rock and a broiler breed, the estimate of heritability for cuticle deposition from a meta-analysis was moderately high (0.38 ± 0.04). In the Rhode Island Red breed, the estimate of the genetic correlation between measurements recorded at early and late times during the egg-laying period was ~ 1. There was no negative genetic correlation between cuticle deposition and production traits. Estimates of the genetic correlation of cuticle deposition with shell color ranged from negative values or 0 in brown-egg layers to positive values in white- or tinted-egg layers. Using the intrinsic fluorescence of tryptophan in the cuticle proteins to quantify the amount of cuticle deposition failed because of complex quenching processes. Tryptophan fluorescence intensity at 330 nm was moderately heritable, but there was no evidence of a non-zero genetic correlation with cuticle deposition. This was complicated furthermore by a negative genetic correlation of fluorescence with color in brown eggs, due to the quenching of tryptophan fluorescence by energy transfer to protoporphyrin pigment. We also confirmed that removal of the cuticle increased reflection of ultraviolet wavelengths from the egg. Conclusions: These results provide additional evidence for the need to incorporate cuticle deposition into breeding programs of egg- and meat-type birds in order to reduce vertical and horizontal transmission of potentially pathogenic organisms and to help improve biosecurity in poultry.

Item Type:Articles
Additional Information:The work was also funded by the BBSRC, Lohmann Tierzucht and Aviagen through the BBSRC LINK grants BB/K0070921/1. The Roslin Institute is funded by a BBSRC Institute Strategic Program Grant BB/P013759/1.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Bain, Professor Maureen
Authors: Dunn, I. C., Woolliams, J. A., Wilson, P. W., Icken, W., Cavero, D., Jones, A. C., Quinlan-Pluck, F., Williams, G. O. S., Olori, V., and Bain, M. M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Genetics Selection Evolution
Publisher:BioMed Central
ISSN:0999-193X
ISSN (Online):1297-9686
Copyright Holders:Copyright © 2019 The Authors
First Published:First published in Genetics Selection Evolution 51(1):25
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
597231Cute-Egg, improvement of eggshell cuticle quality to reduce vertical transmission of zoonotic and pathogenic organismsMaureen BainBiotechnology and Biological Sciences Research Council (BBSRC)BB/K006096/1RI BIODIVERSITY ANIMAL HEALTH & COMPMED