Plasmodium berghei-infection induces volume-regulated anion channel-like activity in human hepatoma cells

Prudêncio, M., Derbyshire, E. T., Marques, C. A. , Krishna, S., Mota, M. M. and Staines, H. M. (2009) Plasmodium berghei-infection induces volume-regulated anion channel-like activity in human hepatoma cells. Cellular Microbiology, 11(10), pp. 1492-1501. (doi: 10.1111/j.1462-5822.2009.01342.x) (PMID:19496788) (PMCID:PMC2774480)

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Abstract

Parasite infection can lead to alterations in the permeability of host plasma membranes. Presented here is the first demonstration that this phenomenon occurs in Plasmodium‐infected liver cells. Using the whole‐cell patch‐clamp technique, volume‐regulated anion channel (VRAC) activity was characterized in Huh‐7 cells (a human hepatoma cell line) before and after infection with Plasmodium berghei. Consistent with the presence of VRACs, hypotonic bath solution induced large ion currents in Huh‐7 cells that rectified outwardly, reversed close to the equilibrium potential for Cl‐ and were inhibited by tamoxifen, clomiphene, mefloquine and 5‐nitro‐2, 3‐(phenylpropylamino)‐benzoic acid (NPPB), with IC50 values of 4 ± 1, 4 ± 2, 2 ± 1 and 52 ± 12 μM respectively. In isotonic conditions, initial current recordings measured in uninfected and immature (24 h post invasion) parasite‐infected Huh‐7 cells were similar (with conductances of 14 ± 3 versus 19 ± 5 pS/pF). However, in mature (48–72 h post invasion) parasite‐infected Huh‐7 cells there was a sevenfold increase in currents (with a conductance of 98 ± 16 pS/pF). The elevated currents observed in the latter are consistent with VRAC‐like activity and the possible reasons for their activation are discussed.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:De Almeida Marques, Dr Catarina
Authors: Prudêncio, M., Derbyshire, E. T., Marques, C. A., Krishna, S., Mota, M. M., and Staines, H. M.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Cellular Microbiology
Publisher:Wiley
ISSN:1462-5814
ISSN (Online):1462-5822
Published Online:03 June 2009

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