In vivo generated Th1 cells can migrate to B cell follicles to support B cell responses

Smith, K. M., Brewer, J. M. , Rush, C. M., Riley, J. and Garside, P. (2004) In vivo generated Th1 cells can migrate to B cell follicles to support B cell responses. Journal of Immunology, 173(3), pp. 1640-1646. (doi: 10.4049/jimmunol.173.3.1640) (PMID:15265892)

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Abstract

The description of Th1 and Th2 T cell subsets rationalized the inverse correlation between humoral and cell-mediated immunity. Although Th1 cells were described to support cell-mediated immune responses, their role in supporting certain B cell responses was firmly established. However, there is now a prevailing preconception that provision of B cell help is entirely the domain of Th2 cells and that Th1 cells lack this capacity. Previous studies demonstrated that immunization using aluminum hydroxide adjuvants induces Ag-specific Th2 responses, whereas incorporation of IL-12 with aluminum hydroxide produces a Th1 inducing adjuvant. By immunizing TCR transgenic recipient mice in this fashion, we have generated Ag-specific, traceable Th1 and Th2 cells in vivo and assessed their follicular migration and ability to support B cell responses. In this study we have shown that in vivo polarized Th1 and Th2 cells clonally expand to similar levels and migrate into B cell follicles in which they support B cell responses to a similar degree. Critically, we present direct evidence that in vivo polarized, IFN-γ secreting Th1 cells migrate into B cell follicles where they can interact with Ag-specific B cells.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Garside, Professor Paul and Brewer, Professor James and Smith, Ms Karen and Rush, Dr Catherine
Authors: Smith, K. M., Brewer, J. M., Rush, C. M., Riley, J., and Garside, P.
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Immunology
Publisher:American Association of Immunologists
ISSN:0022-1767
ISSN (Online):1550-6606
Published Online:20 July 2004

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