Detection of arsenical drug resistance in Trypanosoma brucei with a simple fluorescence test

Stewart, M., Krishna, S., Burchmore, R. , Brun, R., de Koning, H. , Boykin, D., Tidwell, R., Hall, J. and Barrett, M. (2005) Detection of arsenical drug resistance in Trypanosoma brucei with a simple fluorescence test. Lancet, 366, pp. 486-487. (doi:10.1016/S0140-6736(05)66793-1)

Full text not currently available from Enlighten.

Abstract

The resurgence of human African trypanosomiasis (HAT), coupled with an increased incidence of drug resistance, is of concern. We report a quick, simple, and sensitive test for identification of parasites resistant to melarsoprol, the main drug used to treat late stage HAT. Resistant parasites are defective in a plasma membrane transporter responsible for drug uptake. The same transporter carries the fluorescent diamidine DB99 (2,5-bis-(4-amidinophenyl)-3,4-dimethylfuran) into trypanosomes. The two DNA-containing structures in the trypanosome—the nucleus and the kinetoplast—begin to fluoresce within 1 min of introduction of DB99, unless drug resistant.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Burchmore, Dr Richard and De Koning, Professor Harry and Barrett, Professor Michael
Authors: Stewart, M., Krishna, S., Burchmore, R., Brun, R., de Koning, H., Boykin, D., Tidwell, R., Hall, J., and Barrett, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Lancet
Publisher:The Lancet Publishing Group
ISSN:0140-6736
ISSN (Online):1474-547X
Related URLs:

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
386141Inhibitors of trypanosomal 6-phosphogluconate dehydrogenase - modes of action and drug delivery strategiesMichael BarrettWellcome Trust (WELLCOME)075277/A/04/ZInfection Immunity and Inflammation Life Sciences