Label-free microfluidic paper-based electrochemical aptasensor for ultrasensitive and simultaneous multiplexed detection of cancer biomarkers

Wang, Y., Luo, J., Liu, J., Sun, S., Xiong, Y., Ma, Y., Yan, S., Yang, Y., Yin, H. and Cai, X. (2019) Label-free microfluidic paper-based electrochemical aptasensor for ultrasensitive and simultaneous multiplexed detection of cancer biomarkers. Biosensors and Bioelectronics, 136, pp. 84-90. (doi: 10.1016/j.bios.2019.04.032) (PMID:31039491)

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Abstract

Simultaneous detection of multiple tumor biomarkers in body fluids could facilitate early diagnosis of lung cancer, so as to provide scientific reference for clinical treatment. This paper depicted a multi-parameter paper-based electrochemical aptasensor for simultaneous detection of carcinoembryonic antigen (CEA) and neuron-specific enolase (NSE) in a clinical sample with high sensitivity and specificity. The paper-based device was fabricated through wax printing and screen-printing, which enabled functions of sample filtration and sample auto injection. Amino functional graphene (NG)-Thionin (THI)- gold nanoparticles (AuNPs) and Prussian blue (PB)- poly (3,4- ethylenedioxythiophene) (PEDOT)- AuNPs nanocomposites were synthesized respectively. They were used to modify the working electrodes not only for promoting the electron transfer rate, but also for immobilization of the CEA and NSE aptamers. A label-free electrochemical method was adopted, enabling a rapid simple point-of-care testing. Experimental results showed that the proposed multi-parameter aptasensor exhibited good linearity in ranges of 0.01-500 ng mL for CEA (R  = 0.989) and 0.05-500 ng mL for NSE (R  = 0.944), respectively. The limit of detection (LOD) was 2 pg mL for CEA and 10 pg mL for NSE. In addition, the device was evaluated using clinical serum samples and received a good correlation with large electrochemical luminescence (ECL) equipment, which would offer a new platform for early cancer diagnostics, especially in those resource-limit areas.

Item Type:Articles
Additional Information:This work is sponsored by the Key Research Programs (QYZDJ-SSW-SYS015) of Frontier Sciences, CAS, China; the National Key Research and Development Program (2017YFA0205902), China; the NSFC (61527815, 61771452 and 61775216), China.
Keywords:Cancer biomarkers, electrochemical aptasensor, microfluidic paper-based device, multiplexed detection, nanocomposites.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Yin, Professor Huabing
Authors: Wang, Y., Luo, J., Liu, J., Sun, S., Xiong, Y., Ma, Y., Yan, S., Yang, Y., Yin, H., and Cai, X.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Biosensors and Bioelectronics
Publisher:Elsevier
ISSN:0956-5663
ISSN (Online):1873-4235
Published Online:18 April 2019
Copyright Holders:Copyright © 2019 Elsevier B.V.
First Published:First published in Biosensors and Bioelectronics 136: 84-90
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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