Assessing the potential interactions between cellular miRNA and arboviral genomic RNA in the Yellow Fever mosquito, Aedes aegypti

Yen, P.-S., Chen, C.-H., Sreenu, V. , Kohl, A. and Failloux, A.-B. (2019) Assessing the potential interactions between cellular miRNA and arboviral genomic RNA in the Yellow Fever mosquito, Aedes aegypti. Viruses, 11(6), 540. (doi: 10.3390/v11060540) (PMID:31185697) (PMCID:PMC6631873)

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Abstract

Although the role of exogenous small interfering RNA (siRNA) and P-element induced wimpy testis (PIWI)-interacting RNA (piRNA) pathways in mosquito antiviral immunity is increasingly better understood, there is still little knowledge regarding the role of mosquito cellular microRNA (miRNA). Identifying direct interactions between the mosquito miRNAs and the RNA genome of arboviruses and choosing the relevant miRNA candidates to explore resulting antiviral mechanisms are critical. Here, we carried out genomic analyses to identify Aedes aegypti miRNAs that potentially interact with various lineages and genotypes of chikungunya, dengue, and Zika viruses. By using prediction tools with distinct algorithms, several miRNA binding sites were commonly found within different genotypes/and or lineages of each arbovirus. We further analyzed those miRNAs that could target more than one arbovirus, required a low energy threshold to form miRNA-viralRNA (vRNA) complexes, and predicted potential RNA structures using RNAhybrid software. We predicted miRNA candidates that might participate in regulating arboviral replication in Ae. aegypti. Even without any experimental validation, which should be done as a next step, this study can shed further light on the role of miRNA in mosquito innate immunity and targets for future studies.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Vattipally, Dr Sreenu and Kohl, Professor Alain
Authors: Yen, P.-S., Chen, C.-H., Sreenu, V., Kohl, A., and Failloux, A.-B.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
Journal Name:Viruses
Publisher:MDPI
ISSN:1999-4915
ISSN (Online):1999-4915
Published Online:10 June 2019
Copyright Holders:Copyright © 2019 The Authors
First Published:First published in Viruses 11(6): 540
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
737371ZikallianceAlain KohlEuropean Commission (EC)734548MVLS III - CENTRE FOR VIRUS RESEARCH
656551Arbovirus interactions with arthropod hostsAlain KohlMedical Research Council (MRC)MC_UU_12014/8MVLS III - CENTRE FOR VIRUS RESEARCH