Evaluation of cytokine responses against novel Mtb antigens as diagnostic markers for TB disease

Awoniyi, D. O. et al. (2016) Evaluation of cytokine responses against novel Mtb antigens as diagnostic markers for TB disease. Journal of Infection, 73(3), pp. 219-230. (doi:10.1016/j.jinf.2016.04.036) (PMID:27311746)

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Objective: We investigated the accuracy of host markers detected in Mtb antigen-stimulated whole blood culture supernatant in the diagnosis of TB. Methods: Prospectively, blood from 322 individuals with presumed TB disease from six African sites was stimulated with four different Mtb antigens (Rv0081, Rv1284, ESAT-6/CFP-10, and Rv2034) in a 24 h whole blood stimulation assay (WBA). The concentrations of 42 host markers in the supernatants were measured using the Luminex multiplex platform. Diagnostic biosignatures were investigated through the use of multivariate analysis techniques. Results: 17% of the participants were HIV infected, 106 had active TB disease and in 216 TB was excluded. Unstimulated concentrations of CRP, SAA, ferritin and IP-10 had better discriminating ability than markers from stimulated samples. Accuracy of marker combinations by general discriminant analysis (GDA) identified a six analyte model with 77% accuracy for TB cases and 84% for non TB cases, with a better performance in HIV uninfected patients. Conclusions: A biosignature of 6 cytokines obtained after stimulation with four Mtb antigens has moderate potential as a diagnostic tool for pulmonary TB disease individuals and stimulated marker expression had no added value to unstimulated marker performance.

Item Type:Articles
Additional Information:Funding: The African European Tuberculosis Consortium (AE-TBC, www.ae-tbc.eu) was funded by the EDCTP, grant number IP_2009_32040, Principal Investigator: Prof. Gerhard Walzl.
Glasgow Author(s) Enlighten ID:Crampin, Professor Mia
Authors: Awoniyi, D. O., Teuchert, A., Sutherland, J. S., Mayanja-Kizza, H., Howe, R., Mihret, A., Loxton, A. G., Sheehama, J., Kassa, D., Crampin, A. C., Dockrell, H. M., Kidd, M., Rosenkrands, I., Geluk, A., Ottenhoff, T. H.M., Corstjens, P.L.A.M., Chegou, N. N., Walzl, G., Walzl, G., Chegou, N. N., Kriel, M., van der Spuy, G., Loxton, A. G., Stanley, K., Malherbe, S., Mcanda, S., Kriel, B., Phalane, K. G., Essone, P., Sutherland, J. S., Owolabi, O., Sillah, A., Mendy, J., Gindeh, A., Donkor, S., Togun, T., Ota, M., Crampin, A. C., Simukonda, F., Amberbir, A., Chilongo, F., Houben, R., Kassa, D., Gebrezgeabher, A., Mesfin, G., Belay, Y., Gebremichael, G., Alemayehu, Y., van der Vyver, M., Amutenya, F. N., Nelongo, J. N., Monye, L., Sheehama, J. A., Iipinge, S., Mayanja-Kizza, H., Namuganga, A. R., Muzanye, G., Nsereko, M., Peters, P., Howe, R., Mihret, A., Bekele, Y., Tessema, B., Yamuah, L., Ottenhoff, T. H.M., Geluk, A., Franken, K., Corstjens, P. L.A.M., Tjon Kon Fat, E. M., de Dood, C. J., van der Ploeg-van Schip, J. J., Rosenkrands, I., Aagaard, C., Kaufmann, S. H.E., Esterhuyse, M. M., Cliff, J. M., and Dockrell, H. M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Health and Wellbeing > Public Health
Journal Name:Journal of Infection
ISSN (Online):1532-2742
Published Online:14 June 2016
Copyright Holders:Copyright © 2016 The British Infection Association
First Published:First published in Journal of Infection 73(3):219-230
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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