de Souza, W. M., Fumagalli, M. J., de Araujo, J., Ometto, T., Modha, S. , Thomazelli, L. M., Durigon, E. L., Murcia, P. R. and Figueiredo, L. T. M. (2019) Discovery of novel astrovirus and calicivirus identified in ruddy turnstones in Brazil. Scientific Reports, 9, 5556. (doi: 10.1038/s41598-019-42110-3) (PMID:30944402) (PMCID:PMC6447618)
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Abstract
Birds are the natural reservoir of viruses with zoonotic potential, as well as contributing to the evolution, emergence, and dissemination of novel viruses. In this study, we applied a high-throughput screening approach to identify the diversity of viruses in 118 samples of birds captured between October 2006 to October 2010 in the North and Northeast regions of Brazil. We found nearly complete genomes of novel species of astrovirus and calicivirus in cloacal swabs of ruddy turnstones (Arenaria interpres) collected in Coroa do Avião islet, Pernambuco State. These viruses are positive-sense single-stranded RNA with a genome of ~7 to 8 kb, and were designated as Ruddy turnstone astrovirus (RtAstV) and Ruddy turnstone calicivirus (RTCV), respectively. Phylogenetic analysis showed that RtAstV and RTCV grouped in a monophyletic clade with viruses identified from poultry samples (i.e., chicken, goose, and turkey), including viruses associated with acute nephritis in chickens. Attempts of viral propagation in monkey and chicken cell lines for both viruses were unsuccessful. Also, we found genomes related with viral families that infect invertebrates and plants, suggesting that they might be ingested in the birds' diet. In sum, these findings shed new light on the diversity of viruses in migratory birds with the notable characterization of a novel astrovirus and calicivirus.
| Item Type: | Articles |
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| Additional Information: | This work was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo, Brazil (Grant Number. 09/05994-9; 11/13821-7; 13/05485-2 and 13/14929-1, and Scholarships No. 12/24150-9; 15/05778-5; 17/13981-0; 16/01414-1 and 18/09383-3). CNPq– process 141981/2006-7. PRM was supported by the Medical Research Council of the United Kingdom (Grant MC_UU_120/14/9). |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Modha, Ms Sejal and Murcia, Professor Pablo |
| Authors: | de Souza, W. M., Fumagalli, M. J., de Araujo, J., Ometto, T., Modha, S., Thomazelli, L. M., Durigon, E. L., Murcia, P. R., and Figueiredo, L. T. M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Scientific Reports |
| Publisher: | Nature Research |
| ISSN: | 2045-2322 |
| ISSN (Online): | 2045-2322 |
| Copyright Holders: | Copyright © 2019 The Authors |
| First Published: | First published in Scientific Reports 9:5556 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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