Komai-Koma, M., Gilchrist, D. S. and Xu, D. (2009) Direct recognition of LPS by human but not murine CD8+ T cells via TLR4 complex. European Journal of Immunology, 39(6), pp. 1564-1572. (doi: 10.1002/eji.200838866)
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Publisher's URL: http://dx.doi.org/10.1002/eji.200838866
Abstract
LPS comprises a major PAMP and is a key target of the immune system during bacterial infection. While LPS can be recognised by innate immune cells via the TLR4 complex, it is unknown whether T lymphocytes, especially CD8<sup>+</sup> T cells are also capable of doing so. We report here that naïve human CD8<sup>+</sup> T cells, after activation by TCR stimulation, express surface TLR4 and CD14. These activated CD8<sup>+</sup> T cells can then secrete high concentrations of IFN-γ, granzyme and perforin in response to LPS. These effects can be specifically inhibited using siRNA for TLR4. Furthermore, LPS can synergise with IL-12 to polarise the CD8<sup>+</sup> T cells into cytotoxic T-cell 1 (Tc1) that produce IFN-γ but not IL-4, with or without TCR activation. Moreover, CD8<sup>+</sup> CD45RO<sup>+</sup> memory T cells constitutively expressed TLR4 and markedly enhanced IFN-γ production when stimulated with LPS. In contrast, activated murine CD8<sup>+</sup> T cells lack TLR4 and CD14 expression and fail to respond to LPS for proliferation and cytokine production. Thus, human but not murine CD8<sup>+</sup> T cells are able to directly recognise bacterial LPS via LPS receptor complex and TLR4 provides a novel signal for the activation of effector and memory human CD8<sup>+</sup> T cells.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Xu, Dr Damo and Komai-Koma, Dr Mousa and Gilchrist, Dr Derek |
Authors: | Komai-Koma, M., Gilchrist, D. S., and Xu, D. |
College/School: | College of Medical Veterinary and Life Sciences |
Journal Name: | European Journal of Immunology |
Journal Abbr.: | Eur. J. Immunol. |
Publisher: | Wiley-VCH Verlag |
ISSN: | 0014-2980 |
ISSN (Online): | 1521-4141 |
Published Online: | 29 April 2009 |
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