Mapping the metabolism of five amino acids in bloodstream form Trypanosoma brucei using U-13C-labelled substrates and LC-MS

Johnston, K., Kim, D.-H., Kerkhoven, E. J., Burchmore, R. , Barrett, M. P. and Achcar, F. (2019) Mapping the metabolism of five amino acids in bloodstream form Trypanosoma brucei using U-13C-labelled substrates and LC-MS. Bioscience Reports, 39(5), BSR2018160. (doi: 10.1042/BSR20181601) (PMID:31028136) (PMCID:PMC6522824)

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Abstract

The metabolism of the parasite Trypanosoma brucei has been the focus of numerous studies since the 1940s. Recently it was shown, using metabolomics coupled with heavy-atom isotope labelled glucose, that the metabolism of the bloodstream form parasite is more complex than previously thought. The present study also raised a number of questions regarding the origin of several metabolites, for example succinate, only a proportion of which derives from glucose. In order to answer some of these questions and explore the metabolism of bloodstream form T. brucei in more depth we followed the fate of five heavy labelled amino acids – glutamine, proline, methionine, cysteine and arginine – using an LC–MS based metabolomics approach. We found that some of these amino acids have roles beyond those previously thought and we have tentatively identified some unexpected metabolites which need to be confirmed and their function determined.

Item Type:Articles
Additional Information:This work was supported by the Leverhulme Trust [grant number ECF-2015-392 (to FA)]; the Wellcome Trust core grant to the Wellcome Centre for Integrative Parasitology [grant number 104111/Z/14/Z (to MPB)]; and the Doctoral Training Centre (DTC) in Technologies at the Interface between Engineering, the Physical Sciences and the Life Sciences, University of [grant number EP/F500424/1 (to KJ)]. The DTC is funded by the Engineering and Physical Sciences Research Council (EPSRC) and the Biotechnology and Biological Sciences Research Council (BBSRC).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Achcar, Dr Fiona and Burchmore, Dr Richard and Kerkhoven, Mr Eduard and Barrett, Professor Michael
Authors: Johnston, K., Kim, D.-H., Kerkhoven, E. J., Burchmore, R., Barrett, M. P., and Achcar, F.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Bioscience Reports
Publisher:Portland Press Ltd.
ISSN:0144-8463
ISSN (Online):1573-4935
Published Online:26 April 2019
Copyright Holders:Copyright © 2019 The Authors
First Published:First published in Bioscience Reports 39(5):BSR2018160
Publisher Policy:Reproduced under a Creative Commons license

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
371799The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOTR)104111/Z/14/Z & AIII - PARASITOLOGY