Human cytomegalovirus genomes sequenced directly from clinical material: variation, multiple-strain infection, recombination and gene loss

Suárez, N. M. et al. (2019) Human cytomegalovirus genomes sequenced directly from clinical material: variation, multiple-strain infection, recombination and gene loss. Journal of Infectious Diseases, 220(5), pp. 781-791. (PMID:31050742) (PMCID:PMC6667795)

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Abstract

The genomic characteristics of human cytomegalovirus (HCMV) strains sequenced directly from clinical pathology samples were investigated, focusing on variation, multiple-strain infection, recombination, and gene loss. A total of 207 datasets generated in this and previous studies using target enrichment and high-throughput sequencing were analyzed, in the process enabling the determination of genome sequences for 91 strains. Key findings were that (i) it is important to monitor the quality of sequencing libraries in investigating variation; (ii) many recombinant strains have been transmitted during HCMV evolution, and some have apparently survived for thousands of years without further recombination; (iii) mutants with nonfunctional genes (pseudogenes) have been circulating and recombining for long periods and can cause congenital infection and resulting clinical sequelae; and (iv) intrahost variation in single-strain infections is much less than that in multiple-strain infections. Future population-based studies are likely to continue illuminating the evolution, epidemiology, and pathogenesis of HCMV.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Camiolo, Dr Salvatore and Wilkie, Dr Gavin and Suarez, Dr Nicolas and Hughes, Dr Joseph and Vattipally, Dr Sreenu and Holton, Dr Mary and Davison, Professor Andrew
Authors: Suárez, N. M., Wilkie, G. S., Hage, E., Camiolo, S., Holton, M., Hughes, J., Maabar, M., Sreenu, V. B., Dhingra, A., Gompels, U. A., Wilkinson, G. W.G., Baldanti, F., Furione, M., Lilleri, D., Arossa, A., Ganzenmueller, T., Gerna, G., Hubáček, P., Schulz, T. F., Wolf, D., Zavattoni, M., and Davison, A. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Infectious Diseases
Publisher:Oxford University Press
ISSN:0022-1899
ISSN (Online):1537-6613
Published Online:02 May 2019
Copyright Holders:Copyright © 2019 The Authors
First Published:First published in Journal of Infectious Diseases 220(5):781-791
Publisher Policy:Reproduced under a Creative Commons license

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
656321Genomics of human cytomegalovirusAndrew DavisonMedical Research Council (MRC)MC_UU_12014/3MVLS III - CENTRE FOR VIRUS RESEARCH
MC_UU_12014/12
738561Exploiting a human challenge model to understand the pathogenesis of cytomegalovirusAndrew DavisonWellcome Trust (WELLCOTR)204870/Z/16/ZMVLS III - CENTRE FOR VIRUS RESEARCH