Identification of nonepithelial multipotent cells in the embryonic olfactory mucosa

Tome, M., Lindsay, S. L., Riddell, J. S. and Barnett, S. C. (2009) Identification of nonepithelial multipotent cells in the embryonic olfactory mucosa. Stem Cells, 27(9), pp. 2196-2208. (doi:10.1002/stem.130) (PMID:19544421)

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Abstract

Olfactory mucosal (OM) tissue, a potential source of stem cells, is currently being assessed in the clinic as a candidate tissue for transplant-mediated repair of spinal cord injury. We examined the ability of embryonic rat OM tissue to generate stem cells using culture conditions known to promote neural stem cell proliferation. Primary spheres formed that proliferated and exhibited two main morphologies: (a) CNS neurosphere-like (OM-I) and (b) small, tight spheroid-like (OM-II). The OM-I spheres expressed the neural stem cell marker nestin but also markers of peripheral glia, neurons, and connective tissue. Further studies demonstrated the presence of multipotential mesenchymal-like stem cells within OM-I spheres that differentiated into bone, adipose, and smooth muscle cells. In contrast, the OM-II spheres contained mainly cytokeratin-expressing cells. Immunolabeling of rat olfactory tissue with Stro-1, CD90, and CD105 showed the presence of multipotent mesenchymal cells in the lamina propria, whereas cytokeratin was expressed by the epithelial cells of the olfactory epithelium. In addition, a comparable pattern of immunoreactivity was detected in human tissue using Stro-1 and cytokeratin, suggesting the presence of similar cells in this tissue. The identification of a nonepithelial multipotent cell in the OM may explain the varied reports on olfactory stem cell differentiation capacity in vitro and in vivo and illustrates the cellular complexity of this tissue as a potential source of stem cells for transplantation and translation to the clinic.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Riddell, Dr John and Barnett, Professor Susan and Lindsay, Dr Susan and Tome, Dr Mercedes
Authors: Tome, M., Lindsay, S. L., Riddell, J. S., and Barnett, S. C.
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Stem Cells
Publisher:AlphaMed Press, Inc.
ISSN:1066-5099
ISSN (Online):1549-4918
Published Online:21 May 2009

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
370981The use of olfactory epithelial stem cells for CNS repairSusan BarnettMedical Research Council (MRC)G0300285Infection Immunity and Inflammation Medicine