3D gelatin-chitosan hybrid hydrogels combined with human platelet lysate highly support human mesenchymal stem cell proliferation and osteogenic differentiation

Re, F. et al. (2019) 3D gelatin-chitosan hybrid hydrogels combined with human platelet lysate highly support human mesenchymal stem cell proliferation and osteogenic differentiation. Journal of Tissue Engineering, 10, pp. 1-16. (doi:10.1177/2041731419845852) (PMID:31105928) (PMCID:PMC6507314)

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Abstract

Bone marrow and adipose tissue human mesenchymal stem cells were seeded in highly performing 3D gelatin–chitosan hybrid hydrogels of varying chitosan content in the presence of human platelet lysate and evaluated for their proliferation and osteogenic differentiation. Both bone marrow and adipose tissue human mesenchymal stem cells in gelatin–chitosan hybrid hydrogel 1 (chitosan content 8.1%) or gelatin–chitosan hybrid hydrogel 2 (chitosan 14.9%) showed high levels of viability (80%–90%), and their proliferation and osteogenic differentiation was significantly higher with human platelet lysate compared to fetal bovine serum, particularly in gelatin–chitosan hybrid hydrogel 1. Mineralization was detected early, after 21 days of culture, when human platelet lysate was used in the presence of osteogenic stimuli. Proteomic characterization of human platelet lysate highlighted 59 proteins mainly involved in functions related to cell adhesion, cellular repairing mechanisms, and regulation of cell differentiation. In conclusion, the combination of our gelatin–chitosan hybrid hydrogels with hPL represents a promising strategy for bone regenerative medicine using human mesenchymal stem cells.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel and Re, Federica and Cantini, Dr Marco
Authors: Re, F., Cantini, M., Almici, C., Bianchetti, A., Chinello, C., Dey, K., Agnelli, S., Manferdini, C., Bernardi, S., Lopomo, N. F., Sardini, E., Borsani, E., Rodella, L. F., Savoldi, F., Paganelli, C., Guizzi, P., Lisignoli, G., Magni, F., Salmeron-Sanchez, M., and Russo, D.
College/School:College of Science and Engineering
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Journal of Tissue Engineering
Publisher:SAGE
ISSN:2041-7314
ISSN (Online):2041-7314
Published Online:02 May 2019
Copyright Holders:Copyright © 2019 The Authors
First Published:First published in Journal of Tissue Engineering 10:1-16
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
722061Engineering growth factor microenvironments- a new therapeutic paradigm for regenerative medicineManuel Salmeron-SanchezEngineering and Physical Sciences Research Council (EPSRC)EP/P001114/1ENG - BIOMEDICAL ENGINEERING
3036130Engineered microenvironments to harvest stem cell response to viscosity for cartilage repairMarco CantiniMedical Research Council (MRC)MR/S005412/1ENG - Biomedical Engineering