Detection of changes in mitochondrial hydrogen sulfide (H2S) in vivo in the fish model Poecilia mexicana (Poeciliidae)

Lau, G. Y., Barts, N., Hartley, R. C. , Tobler, M., Richards, J. G., Murphy, M. P. and Arndt, S. (2019) Detection of changes in mitochondrial hydrogen sulfide (H2S) in vivo in the fish model Poecilia mexicana (Poeciliidae). Biology Open, 8, bio041467. (doi: 10.1242/bio.041467) (PMID:31072908) (PMCID:PMC6550084)

Abstract

In this paper, we outline the use of a mitochondria-targeted ratiometric mass spectrometry probe, MitoA, to detect in vivo changes in mitochondrial hydrogen sulfide (H2S) in Poecilia mexicana (family Poeciliidae). MitoA is introduced via intraperitoneal injection into the animal and is taken up by mitochondria, where it reacts with H2S to form the product MitoN. The MitoN/MitoA ratio can be used to assess relative changes in the amounts of mitochondrial H2S produced over time. We describe the use of MitoA in the fish species P. mexicana to illustrate the steps for adopting the use of MitoA in a new organism, including extraction and purification of MitoA and MitoN from tissues followed by tandem mass spectrometry. In this proof-of-concept study we exposed H2S tolerant P. mexicana to 59 µM free H2S for 5 h, which resulted in increased MitoN/MitoA in brain and gills, but not in liver or muscle, demonstrating increased mitochondrial H2S levels in select tissues following whole-animal H2S exposure. This is the first time that accumulation of H2S has been observed in vivo during whole-animal exposure to free H2S using MitoA.

• Item Type: Articles
• Additional Information: This work was supported by grants from the National Science Foundation (IOS-1557860), the U.S. Army Research Office (W911NF-15-1-0175) and the Defense University Research Instrumentation Program of the U.S. Office of Naval Research (W911NF-16-1-0225) to M.T.; by a Natural Sciences and Engineering Research Council (NSERC) of Canada Discovery Grant to J.G.R.; and by the Medical Research Council UK (MC_U105663142) and a Wellcome Trust Investigator award (110159/Z/15/Z) to M.P.M. G.Y.L. was supported by a NSERC Canada Graduate Scholarship.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Hartley, Professor Richard

Creator Roles

Hartley, R. C.: Resources

• Authors: Lau, G. Y., Barts, N., Hartley, R. C., Tobler, M., Richards, J. G., Murphy, M. P., and Arndt, S.
• College/School: College of Science and Engineering > School of Chemistry
• Journal Name: Biology Open
• Publisher: Company of Biologists
• ISSN: 2046-6390
• ISSN (Online): 2046-6390
• Published Online: 09 May 2019
• Copyright Holders: Copyright © 2019. Published by The Company of Biologists Ltd
• First Published: First published in Biology Open 8:bio041467
• Publisher Policy: Reproduced under a Creative Commons license