Identification of thermotolerant Cronobacter strains using multiplex real-time polymerase chain reaction

Kaclíková, E. and Oravcová, K. (2016) Identification of thermotolerant Cronobacter strains using multiplex real-time polymerase chain reaction. Journal of Food and Nutrition Research, 55(3), pp. 278-281.

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Abstract

Cronobacter sp. is considered an opportunistic pathogen implicated in food-borne life-threatening infection, in particular in neonates and infants. Contaminated powdered infant formula was identified as the primary source of Cronobacter sp. at very low levels. Multiplication of survived cells of thermotolerant Cronobacter strains was often found a crucial factor for causing outbreaks. The aim of our work was to develop and evaluate a detection method for thermotolerant Cronobacter strains using triplex TaqMan real-time polymerase chain reaction (PCR) targeting a specific sequence for Cronobacter sp., a thermotolerance marker and an internal amplification control. The proposed method was evaluated on 48 strains, including 32 Cronobacter sp. and 16 related species of Enterobacteriaceae family. The results showed 100% inclusivity and exclusivity, and 100% correlation with the time required to kill 90% of cells exposed at 58 °C (D58-value). The developed real-time PCR method facilitated rapid and specific detection of thermotolerant Cronobacter strains isolated from powdered infant formula products or from the production chain.

Item Type:Articles
Additional Information:This work is a result of implementation of the project ITMS 26240220089 “Effective control methods for safety foods” of the Agency for the Structural Funds of the European Union, Ministry of Education, Science, Research and Sport of the Slovak Republic.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Oravcova, Dr Katarina
Authors: Kaclíková, E., and Oravcová, K.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Journal of Food and Nutrition Research
Publisher:Vup Food Research Institute
ISSN:1336-8672
ISSN (Online):2333-1119
Published Online:26 May 2016
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