Biofilm formation in various conditions is not a key factor of persistence potential if Listeria monocytogenes in food-processing environment

Koreňová, J., Oravcová, K. , Véghová, A., Karpíšková, R. and Kuchta, T. (2016) Biofilm formation in various conditions is not a key factor of persistence potential if Listeria monocytogenes in food-processing environment. Journal of Food and Nutrition Research, 55(2), pp. 189-193.

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Abstract

In this study, a hypothesis was tested that biofilm formation ability, or ability to form biofilm in specific conditions, is responsible for persistence of Listeria monocytogenes in food processing environment. Thirty-three L. monocytogenes strains isolated from food-processing environment and food products were tested for biofilm-forming ability in various conditions. Biofilm-forming ability was tested with exponential-phase cultures at 20 °C, 37 °C and 40 °C, with stationaryphase cultures at 20 °C, 37 °C and 40 °C, with cultures grown in limited-nutrient conditions and with cultures grown in high-salt conditions at 4 °C. Biofilm was formed in 96-well polystyrene plates and quantified by the method based on staining with crystal violet. The results showed that biofilm-forming ability of L. monocytogenes strains was variable, individual strains exhibiting different relative biofilm-forming ability in various conditions. No statistically significant differences between the groups of persistent and sporadic strains in biofilm-forming ability in any of the conditions were determined. Based on our results, biofilm-forming ability of L. monocytogenes is not a key factor that determines its persistence potential in food-processing environment.

Item Type:Articles
Additional Information:This work is a result of implementation of the project ITMS 26240220089 “Effective control methods for safety foods” of the Agency for the Structural Funds of the European Union, Ministry of Education, Science, Research and Sport of the Slovak Republic.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Oravcova, Dr Katarina
Authors: Koreňová, J., Oravcová, K., Véghová, A., Karpíšková, R., and Kuchta, T.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Journal of Food and Nutrition Research
Publisher:Vup Food Research Institute
ISSN:1336-8672
ISSN (Online):2333-1119
Published Online:16 May 2016
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