Xia, L. , Marques-Bueno, M. M., Bruce, C. G. and Karnik, R. (2019) Unusual roles of secretory SNARE SYP132 in plasma membrane H+-ATPase traffic and vegetative plant growth. Plant Physiology, 180(2), pp. 837-858. (doi: 10.1104/pp.19.00266) (PMID:30926657)
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183282.pdf - Accepted Version 17MB |
Abstract
The plasma membrane proton (H+)-ATPases of plants generate steep electrochemical gradients and activate osmotic solute uptake. H+-ATPase-mediated proton pumping orchestrates cellular homeostasis and is a prerequisite for plastic cell expansion and plant growth. All evidence suggests that the population of H+-ATPase proteins at the plasma membrane reflects a balance of their roles in exocytosis, endocytosis, and recycling. Auxin governs both traffic and activation of the plasma membrane H+-ATPase proteins already present at the membrane. As in other eukaryotes, in plants, SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor)-mediated membrane traffic influences the density of several proteins at the plasma membrane. Even so, H+-ATPase traffic, its relationship with SNAREs, and its regulation by auxin have remained enigmatic. Here, we identify the Arabidopsis (Arabidopsis thaliana) Qa-SNARE SYP132 (Syntaxin of Plants 132) as a key factor in H+-ATPase traffic and demonstrate its association with endocytosis. SYP132 is a low abundant, secretory SNARE that primarily localizes to the plasma membrane. We find that SYP132 expression is tightly regulated by auxin and that augmented SYP132 expression reduces the amount of H+-ATPase proteins at the plasma membrane. The physiological consequences of SYP132 over-expression include reduced apoplast acidification and suppressed vegetative growth. Thus, SYP132 plays unexpected and vital roles in auxin-regulated H+-ATPase traffic and associated functions at the plasma membrane.
| Item Type: | Articles |
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| Additional Information: | This work was supported by the Royal Society University Research Fellowship UF150364 and Royal Society research grant RG160493 to RK. LX is funded by a PhD scholarship from the China Scholarship Council. CB and MMB were supported by the University of Glasgow Leadership funds to RK |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Marques-Bueno, Dr Maria Del Mar and Karnik, Dr Rucha and Bruce, Mr Craig and Xia, Mr Lingfeng |
| Authors: | Xia, L., Marques-Bueno, M. M., Bruce, C. G., and Karnik, R. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | Plant Physiology |
| Publisher: | American Society of Plant Biologists |
| ISSN: | 0032-0889 |
| ISSN (Online): | 1532-2548 |
| Published Online: | 29 March 2019 |
| Copyright Holders: | Copyright © 2019 The American Society of Plant Biologists |
| First Published: | First published in Plant Physiology 180(2):837-858 |
| Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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