Vasomotor and fibrinolytic responses to kinin receptor agonists in the atherosclerotic human lower limb

Cruden, N. L.M., Lang, N. N. , MacGillivray, T. J., Uren, N. G., Fox, K. A.A. and Newby, D. E. (2012) Vasomotor and fibrinolytic responses to kinin receptor agonists in the atherosclerotic human lower limb. Heart and Vessels, 27(2), pp. 179-185. (doi: 10.1007/s00380-011-0124-6) (PMID:21394642)

Abstract

Upregulation of vascular B1 kinin receptor expression has been reported in human atheroma, but its role remains unclear. We examined vasomotor and fibrinolytic responses to selective B1 and B2 kinin receptor agonism in the human femoral circulation and correlated responses with femoral arterial plaque load. Femoral arterial cross-sectional area, blood flow and plaque volume were determined using intravascular ultrasound and Doppler during selective arterial infusion of Lys-des-Arg9-bradykinin (B1 agonist), bradykinin (B2 agonist) and sodium nitroprusside in eleven patients undergoing diagnostic coronary angiography. Net release of tissue plasminogen activator was determined across the femoral vascular bed. Mean femoral arterial plaque load was 8.1 (±0.9) mm3/mm of vessel. Bradykinin and sodium nitroprusside caused dose-dependent increases in femoral blood flow (p < 0.05 and p < 0.005, respectively). Bradykinin caused a dose-dependent increase in net tissue plasminogen activator release (p < 0.05), which was augmented by angiotensin-converting enzyme inhibition (p < 0.05). There were no correlations between plaque load and bradykinin-mediated vasodilation or tissue plasminogen activator release. Lys-des-Arg9-bradykinin had no effect on blood flow or tissue plasminogen activator release. The vasomotor and fibrinolytic actions of bradykinin in the femoral circulation are mediated solely by the B2 kinin receptor, irrespective of the presence of atheroma. In keeping with previous data, bradykinin-mediated tissue plasminogen activator release was augmented in the presence of angiotensin-converting enzyme inhibition consistent with its putative vascular protective effect.

• Item Type: Articles
• Additional Information: This work was funded by the British Heart Foundation (PG/02/092).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Newby, Professor David and Lang, Professor Ninian
• Authors: Cruden, N. L.M., Lang, N. N., MacGillivray, T. J., Uren, N. G., Fox, K. A.A., and Newby, D. E.
• College/School: College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
• Journal Name: Heart and Vessels
• Publisher: Springer
• ISSN: 0910-8327
• ISSN (Online): 1615-2573
• Published Online: 11 March 2011