SNAREs SYP121 and SYP122 mediate the secretion of distinct cargo subsets

Waghmare, S., Lileikyte, E., Karnik, R. , Goodman, J. K., Blatt, M. R. and Jones, A. M.E. (2018) SNAREs SYP121 and SYP122 mediate the secretion of distinct cargo subsets. Plant Physiology, 178(4), pp. 1679-1688. (doi:10.1104/pp.18.00832) (PMID:30348815)

Waghmare, S., Lileikyte, E., Karnik, R. , Goodman, J. K., Blatt, M. R. and Jones, A. M.E. (2018) SNAREs SYP121 and SYP122 mediate the secretion of distinct cargo subsets. Plant Physiology, 178(4), pp. 1679-1688. (doi:10.1104/pp.18.00832) (PMID:30348815)

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Abstract

SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins drive vesicle fusion and contribute to homoeostasis, pathogen defense, cell expansion, and growth in plants. In Arabidopsis (Arabidopsis thaliana), two homologous Qa-SNAREs, SYNTAXIN OF PLANTS121 (SYP121) and SYP122, facilitate the majority of secretory traffic to the plasma membrane, and the single mutants are indistinguishable from wild-type plants in the absence of stress, implying a redundancy in their functions. Nonetheless, several studies suggest differences among the secretory cargo of these SNAREs. To address this issue, we conducted an analysis of the proteins secreted by cultured wild-type, syp121, and syp122 mutant Arabidopsis seedlings. Here, we report that a number of cargo proteins were associated differentially with traffic mediated by SYP121 and SYP122. The data also indicated important overlaps between the SNAREs. Therefore, we conclude that the two Qa-SNAREs mediate distinct but complementary secretory pathways during vegetative plant growth.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Lileikyte, Miss Edita and Karnik, Dr Rucha and Blatt, Professor Michael and Waghmare, Dr Sakharam
Authors: Waghmare, S., Lileikyte, E., Karnik, R., Goodman, J. K., Blatt, M. R., and Jones, A. M.E.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Plant Physiology
Publisher:American Society of Plant Biologists
ISSN:0032-0889
ISSN (Online):1532-2548
Published Online:22 October 2018
Copyright Holders:Copyright © 2018 American Society of Plant Biologists

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
703441Dissecting a new and vital checkpoint in SNARE recycling and plant growthMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/N006909/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
656891Analysing GORK clustering for enhanced stomatal controlMichael BlattBiotechnology and Biological Sciences Research Council (BBSRC)BB/M001601/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
696911NEURICEMichael BlattEuropean Commission (EC)678168RI MOLECULAR CELL & SYSTEMS BIOLOGY