Use of in vivo phage display to engineer novel adenoviruses for targeted delivery to the cardiac vasculature

Nicol, C. G., Denby, L., Lopez-Franco, O., Masson, R., Halliday, C. A., Nicklin, S. A., Kritz, A., Work, L. M. and Baker, A. H. (2009) Use of in vivo phage display to engineer novel adenoviruses for targeted delivery to the cardiac vasculature. FEBS Letters, 583(12), pp. 2100-2107. (doi:10.1016/j.febslet.2009.05.037)

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Publisher's URL: http://dx.doi.org/10.1016/j.febslet.2009.05.037

Abstract

We performed in vivo phage display in the stroke prone spontaneously hypertensive rat, a cardiovascular disease model, and the normotensive Wistar Kyoto rat to identify cardiac targeting peptides, and then assessed each in the context of viral gene delivery. We identified both common and strain-selective peptides, potentially indicating ubiquitous markers and those found selectively in dysfunctional microvasculature of the heart. We show the utility of the peptide, DDTRHWG, for targeted gene delivery in human cells and rats in vivo when cloned into the fiber protein of subgroup D adenovirus 19p. This study therefore identifies cardiac targeting peptides by in vivo phage display and the potential of a candidate peptide for vector targeting strategies.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Lopez-Franco, Dr Oscar and Halliday, Dr Crawford and Nicklin, Professor Stuart and Nicol, Dr Campbell and Shirley, Dr Rachel and Baker, Professor Andrew and Denby, Dr Laura and Work, Dr Lorraine and Kritz Wilson, Dr Angelika
Authors: Nicol, C. G., Denby, L., Lopez-Franco, O., Masson, R., Halliday, C. A., Nicklin, S. A., Kritz, A., Work, L. M., and Baker, A. H.
Subjects:Q Science > QH Natural history > QH345 Biochemistry
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:FEBS Letters
Publisher:Elsevier
ISSN:0014-5793
ISSN (Online):1873-3468
Published Online:28 May 2009
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
388211Targeted gene therapy for cardiovascular diseaseAndrew BakerMedical Research Council (MRC)G0400052Institute of Cardiovascular and Medical Sciences