Nicol, C. G., Denby, L., Lopez-Franco, O., Masson, R., Halliday, C. A., Nicklin, S. A., Kritz, A., Work, L. M. and Baker, A. H. (2009) Use of in vivo phage display to engineer novel adenoviruses for targeted delivery to the cardiac vasculature. FEBS Letters, 583(12), pp. 2100-2107. (doi: 10.1016/j.febslet.2009.05.037)
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Publisher's URL: http://dx.doi.org/10.1016/j.febslet.2009.05.037
Abstract
We performed in vivo phage display in the stroke prone spontaneously hypertensive rat, a cardiovascular disease model, and the normotensive Wistar Kyoto rat to identify cardiac targeting peptides, and then assessed each in the context of viral gene delivery. We identified both common and strain-selective peptides, potentially indicating ubiquitous markers and those found selectively in dysfunctional microvasculature of the heart. We show the utility of the peptide, DDTRHWG, for targeted gene delivery in human cells and rats in vivo when cloned into the fiber protein of subgroup D adenovirus 19p. This study therefore identifies cardiac targeting peptides by in vivo phage display and the potential of a candidate peptide for vector targeting strategies.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Lopez-Franco, Dr Oscar and Nicklin, Professor Stuart and Masson, Dr Rachel and Baker, Professor Andrew and Denby, Dr Laura and Halliday, Dr Crawford and Nicol, Dr Campbell and Work, Dr Lorraine and Kritz Wilson, Dr Angelika |
| Authors: | Nicol, C. G., Denby, L., Lopez-Franco, O., Masson, R., Halliday, C. A., Nicklin, S. A., Kritz, A., Work, L. M., and Baker, A. H. |
| Subjects: | Q Science > QH Natural history > QH345 Biochemistry |
| College/School: | College of Medical Veterinary and Life Sciences College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health |
| Journal Name: | FEBS Letters |
| Publisher: | Elsevier |
| ISSN: | 0014-5793 |
| ISSN (Online): | 1873-3468 |
| Published Online: | 28 May 2009 |
| Related URLs: |
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