Secretion of IFN- and not IL-2 by anergic human T cells correlates with assembly of an immature immune synapse

Carlin, L. M. , Yanagi, K., Verhoef, A., Noelte-'t Hoen, E. N.M., Yates, J., Gardner, L., Lamb, J., Lombardi, G., Dallman, M. J. and Davis, D. M. (2005) Secretion of IFN- and not IL-2 by anergic human T cells correlates with assembly of an immature immune synapse. Blood, 106(12), pp. 3874-3879. (doi:10.1182/blood-2005-03-0996) (PMID:16099874)

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Abstract

We report differences in the supramolecular organization of the immunologic synapse (IS) formed by resting and anergic human T cells with agonist peptide-loaded antigen-presenting cells (APCs). T cells reactive to influenza A hemagglutinin peptide or Fel d 1 peptide 4 were rendered both anergic and regulatory by incubation with high doses of agonist peptide in the absence of APCs. At the IS between resting T cells and peptide-loaded APCs, both CD3ϵ and CD3ζ initially accumulate within a ring or arc before redistributing within 30 minutes to single or multiple foci more central to the contact. In contrast, at synapses formed by anergized T cells, CD3ϵ and CD3ζ remained organized within an arc or ring and failed to redistribute centrally. However, intercellular communication between anergic human T cells and agonist peptide-loaded APCs was not a null event, since it triggered secretion of T-cell interferon γ (IFN-γ) but not, for example, interleukin 2 (IL-2). Thus, distinct organizations of CD3 at the T-cell IS correlate with different cytokine profiles; the mature IS formed by resting T cells correlates with their production of both IFN-γ and IL-2, whereas the immature IS formed by anergic T cells seems able to facilitate IFN-γ but not IL-2 production.

Item Type:Articles
Additional Information:Supported by the Biotechnology and Biological Sciences Research Council (BBSRC), the Human Frontier Science Program (HFSP), and Medical Research Council (MRC). J.Y. holds an MRC Training Fellowship.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Carlin, Dr Leo
Authors: Carlin, L. M., Yanagi, K., Verhoef, A., Noelte-'t Hoen, E. N.M., Yates, J., Gardner, L., Lamb, J., Lombardi, G., Dallman, M. J., and Davis, D. M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Blood
Publisher:American Society of Hematology
ISSN:0006-4971
ISSN (Online):1528-0020
Published Online:16 November 2005

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