Measuring intravascular migration of mouse Ly6Clow monocytes in vivo using intravital microscopy

Carlin, L.M. , Auffray, C. and Geissmann, F. (2013) Measuring intravascular migration of mouse Ly6Clow monocytes in vivo using intravital microscopy. Current Protocols in Immunology, 101(1), 14.33.1-14.33.16. (doi: 10.1002/0471142735.im1433s101) (PMID:23564685)

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Abstract

This unit describes methods for intravital imaging of monocytes in the vasculature of the dermis and the mesentery in vivo using fluorescent reporter mice, fluorescent dyes, and antibodies. Cx3cr1gfp/gfp (or +), Rag2−/−, Il2rg−/− mice expressing eGFP at the locus of the Cx3cr1 gene, on the Rag2−/− Il2rg−/− C57Bl/6 background, are used. Although aimed at specifically tracking Ly6Clow monocytes, these protocols could readily be adapted to investigate the interaction of other blood leukocytes with the vascular endothelium by use of other fluorescent reporter mice and fluorescently labeled antibodies.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Carlin, Dr Leo
Authors: Carlin, L.M., Auffray, C., and Geissmann, F.
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Journal Name:Current Protocols in Immunology
Publisher:Wiley
ISSN:1934-3671
ISSN (Online):1934-368X

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