Th17-inducing conditions lead to in vitro activation of both Th17 and Th1 responses in Behcet’s disease

Deniz, R., Tulunay-Virlan, A. , Ture Ozdemir, F., Unal, A. U., Ozen, G., Alibaz-Oner, F., Aydin-Tatli, I., Mumcu, G., Ergun, T. and Direskeneli, H. (2017) Th17-inducing conditions lead to in vitro activation of both Th17 and Th1 responses in Behcet’s disease. Immunological Investigations, 46(5), pp. 518-525. (doi: 10.1080/08820139.2017.1306865) (PMID:28414558)

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Abstract

Objectives: Interleukin-17 (IL-17) has been associated with the pathogenesis of various autoimmune/inflammatory diseases. The aim of this study was to investigate the expression of Th17-related immunity in an innate immunity-dominated vasculitis, namely Behcet’s disease (BD). Methods: Peripheral blood mononuclear cells from 37 patients (age: 38.5 ± 9.8 years) with BD, and 25 healthy controls (HC) (age: 39.1 ± 9.3 years), were cultured in Th17-inducing conditions (IL-6, Phytohemagglutinin (PHA), IL-1β, and IL-23) for 6 days. Cultured cells were stained with CD4, CD8, CD3, TCR gamma/delta, CD19, interferon-γ (IFN-γ), and IL-17 antibodies to determine the intracellular cytokine secretion by flow cytometry. Results: IL-17 expression by CD8+ and γδ+ T cells was higher in BD compared to HC (p = 0.004, p = 0.003, respectively). No differences were observed between the groups in the IL-17 production by B cells. Under Th17-inducing conditions, production of IFN-γ by CD4+, CD8+, and γδ+ T cells was also higher in BD compared to HC (p < 0.05 in all). Conclusion: Our results suggest that under Th17-stimulating conditions, T cells express both IL-17 and IFN-γ in BD. More prominent IL-17 and IFN-γ production by all lymphocyte subsets in BD might be associated with the increased innate responses, early tissue neutrophil infiltrations and late adaptive immunity in BD.

Item Type:Articles
Additional Information:This article was funded by Marmara University Research Fund (BAPKO) (Project No. SAG-A-090512.0132).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Tulunay Virlan, Dr Aysin
Authors: Deniz, R., Tulunay-Virlan, A., Ture Ozdemir, F., Unal, A. U., Ozen, G., Alibaz-Oner, F., Aydin-Tatli, I., Mumcu, G., Ergun, T., and Direskeneli, H.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Immunological Investigations
Publisher:Taylor & Francis
ISSN:0882-0139
ISSN (Online):1532-4311
Published Online:17 April 2017

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