Epithelial function in the Drosophila Malpighian tubule: an in vivo renal model

Davies, S.-A. , Cabrero, P. , Marley, R., Martinez-Corrales, G., Ghimire, S., Dornan, A. J. and Dow, J. A.T. (2019) Epithelial function in the Drosophila Malpighian tubule: an in vivo renal model. In: Vainio, S. (ed.) Kidney Organogenesis: Methods and Protocols. Series: Methods in molecular biology (1926). Humana Press: New York, NY, pp. 203-221. ISBN 9781493990207 (doi: 10.1007/978-1-4939-9021-4_17)

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The insect renal (Malpighian) tubule has long been a model system for the study of fluid secretion and its neurohormonal control, as well as studies on ion transport mechanisms. To extend these studies beyond the boundaries of classical physiology, a molecular genetic approach together with the 'omics technologies is required. To achieve this in any vertebrate transporting epithelium remains a daunting task, as the genetic tools available are still relatively unsophisticated. Drosophila melanogaster, however, is an outstanding model organism for molecular genetics. Here we describe a technique for fluid secretion assays in the D. melanogaster equivalent of the kidney nephron. The development of this first physiological assay for a Drosophila epithelium, allowing combined approaches of integrative physiology and functional genomics, has now provided biologists with an entirely new model system, the Drosophila Malpighian tubule, which is utilized in multiple fields as diverse as kidney disease research and development of new modes of pest insect control.

Item Type:Book Sections
Keywords:Drosophila melanogaster, epithelia, fluid secretion assays, functional genomics, kidney disease model, Malpighian tubules, renal.
Glasgow Author(s) Enlighten ID:Cabrero, Mr Pablo and Dow, Professor Julian and Marley, Dr Richard and Dornan, Dr Anthony and Ghimire, Mr Saurav and Davies, Professor Shireen and Martinez Corrales, Guillermo
Authors: Davies, S.-A., Cabrero, P., Marley, R., Martinez-Corrales, G., Ghimire, S., Dornan, A. J., and Dow, J. A.T.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Publisher:Humana Press
Published Online:12 February 2019

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