Caspase-3 is necessary and sufficient for cleavage of protein synthesis eukaryotic initiation factor 4G during apoptosis

Bushell, M. , McKendrick, L., Jänicke, R. U., Clemens, M. J. and Morley, S. J. (1999) Caspase-3 is necessary and sufficient for cleavage of protein synthesis eukaryotic initiation factor 4G during apoptosis. FEBS Letters, 451(3), pp. 332-336. (doi: 10.1016/S0014-5793(99)00614-6) (PMID:10371215)

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Abstract

Induction of apoptosis BJAB cells is accompanied by the rapid cleavage of protein synthesis eukaryotic initiation factor 4G and the appearance of a fragment of approximately 76 kDa. Inhibition of apoptotic proteases (caspases) has previously been shown to prevent the cleavage of eukaryotic initiation factor 4G. In MCF‐7 breast carcinoma cells, which are deficient in caspase‐3, eukaryotic initiation factor 4G is not cleaved but in vivo expression of caspase‐3 restores eukaryotic initiation factor 4G cleavage following induction of apoptosis. Recombinant caspase‐3 can also cleave eukaryotic initiation factor 4G to yield the 76 kDa fragment both in cell extracts and when the eukaryotic initiation factor 4G is presented in a purified eukaryotic initiation factor 4F complex. These results indicate that caspase‐3 activity is necessary and sufficient for eukaryotic initiation factor 4G degradation.

Item Type:Articles
Additional Information:This research was supported by grants from The Royal Society, The Wellcome Trust, the Cancer Prevention Research Trust and the Leukaemia Research Fund. M.B. is funded by an Industrial CASE Studentship in collaboration with Roche Discovery (Welwyn Garden City) and S.J.M. is a Senior Research Fellow of the Wellcome Trust.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Bushell, Professor Martin
Authors: Bushell, M., McKendrick, L., Jänicke, R. U., Clemens, M. J., and Morley, S. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:FEBS Letters
Publisher:Wiley
ISSN:0014-5793
ISSN (Online):1873-3468

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