Assessment of neuropeptide binding sites and the impact of biostable kinin and CAP2b analogue treatment on aphid (Myzus persicae and Macrosiphum rosae) stress tolerance

Alford, L., Marley, R., Dornan, A. , Pierre, J.-S., Dow, J. A.T. , Nachman, R. J. and Davies, S. A. (2019) Assessment of neuropeptide binding sites and the impact of biostable kinin and CAP2b analogue treatment on aphid (Myzus persicae and Macrosiphum rosae) stress tolerance. Pest Management Science, 75(6), pp. 1750-1759. (doi:10.1002/ps.5372) (PMID:30734498) (PMCID:PMC6593983)

Alford, L., Marley, R., Dornan, A. , Pierre, J.-S., Dow, J. A.T. , Nachman, R. J. and Davies, S. A. (2019) Assessment of neuropeptide binding sites and the impact of biostable kinin and CAP2b analogue treatment on aphid (Myzus persicae and Macrosiphum rosae) stress tolerance. Pest Management Science, 75(6), pp. 1750-1759. (doi:10.1002/ps.5372) (PMID:30734498) (PMCID:PMC6593983)

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Abstract

Background: Neuropeptides are regulators of critical life processes in insects and, due to their high specificity, represent potential targets in the development of greener insecticidal agents. Fundamental to this drive is understanding neuroendocrine pathways that control key physiological processes in pest insects and the screening of potential analogues. The current study investigated neuropeptide binding sites of kinin and CAPA (CAPA‐1) in the aphids Myzus persicae and Macrosiphum rosae and the effect of biostable analogues on aphid fitness under conditions of desiccation, starvation and thermal (cold) stress. Results: M. persicae and M. rosae displayed identical patterns of neuropeptide receptor mapping along the gut, with the gut musculature representing the main target for kinin and CAPA‐1 action. While kinin receptor binding was observed in the brain and VNC of M. persicae, this was not observed in M. rosae. Furthermore, no CAPA‐1 receptor binding was observed in the brain and VNC of either species. CAP2b/PK analogues (with CAPA receptor cross‐activity) were most effective in reducing aphid fitness under conditions of desiccation and starvation stress, particularly analogues 1895 (2Abf‐Suc‐FGPRLa) and 2129 (2Abf‐Suc‐ATPRIa), which expedited aphid mortality. All analogues, with the exception of 2139‐Ac, were efficient at reducing aphid survival under cold stress, although were equivalent in the strength of their effect. Conclusion: In demonstrating the effects of analogues belonging to the CAP2b neuropeptide family and key analogue structures that reduce aphid fitness under stress conditions, this research will feed into the development of second generation analogues and ultimately the development of neuropeptidomimetic‐based insecticidal agents.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Alford, Dr Lucy and Dow, Professor Julian and Davies, Professor Shireen and Dornan, Dr Anthony and Marley, Dr Richard
Authors: Alford, L., Marley, R., Dornan, A., Pierre, J.-S., Dow, J. A.T., Nachman, R. J., and Davies, S. A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Pest Management Science
Publisher:Wiley
ISSN:1526-498X
ISSN (Online):1526-4998
Published Online:07 February 2019
Copyright Holders:Copyright © 2019 The Authors
First Published:First published in Pest Management Science 75(6): 1750-1759
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
171405nEUROSTRESSPEPShireen DaviesEuropean Commission (EC)634361MCSB - Integrative & Systems Biology