The non-proteinogenic amino acids l-methionine sulfoximine and dl-phosphinothricin activate mTOR

Tardito, S. , Chiu, M., Franchi-Gazzola, R., Dall’Asta, V., Comi, P. and Bussolati, O. (2012) The non-proteinogenic amino acids l-methionine sulfoximine and dl-phosphinothricin activate mTOR. Amino Acids, 42(6), pp. 2507-2512. (doi: 10.1007/s00726-011-0981-4) (PMID:21769496)

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Abstract

l-Methionine sulfoximine (MSO) and dl-Phosphinothricin (PPT), two non-proteinogenic amino acids known as inhibitors of Glutamine Synthetase, cause a dose-dependent increase in the phosphorylation of the mTOR substrate S6 kinase 1. The effect is particularly evident in glutamine-depleted cells, where mTOR activity is very low, but is detectable for PPT also in the presence of glutamine. The stimulation of mTOR activity by either MSO or PPT is strongly synergized by essential amino acids. Thus, the non-proteinogenic amino acids MSO and PPT are mTOR activators.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Tardito, Dr Saverio
Authors: Tardito, S., Chiu, M., Franchi-Gazzola, R., Dall’Asta, V., Comi, P., and Bussolati, O.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Amino Acids
Publisher:Springer
ISSN:0939-4451
ISSN (Online):1438-2199
Published Online:17 July 2011

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