Glutamine stimulates mTORC1 independent of the cell content of essential amino acids

Chiu, M., Tardito, S. , Barilli, A., Bianchi, M. G., Dall’Asta, V. and Bussolati, O. (2012) Glutamine stimulates mTORC1 independent of the cell content of essential amino acids. Amino Acids, 43(6), pp. 2561-2567. (doi: 10.1007/s00726-012-1312-0) (PMID:22566039)

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Abstract

Glutamine and leucine are important mTORC1 modulators, although their roles are not precisely defined. In HepG2 and HeLa cells glutamine-free incubation lowers mTORC1 activity, although cell leucine is not decreased. mTORC1 activity, suppressed by amino acid-free incubation, is completely rescued only if essential amino acids (EAA) and glutamine are simultaneously restored, although cell leucine is higher in the absence than in the presence of glutamine. Thus, glutamine stimulates mTORC1 independent of cell leucine, suggesting the existence of two distinct stimulatory signals from either glutamine or EAA.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Tardito, Dr Saverio
Authors: Chiu, M., Tardito, S., Barilli, A., Bianchi, M. G., Dall’Asta, V., and Bussolati, O.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Amino Acids
Publisher:Springer
ISSN:0939-4451
ISSN (Online):1438-2199
Published Online:08 May 2012

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