Differentiation of adult-type Leydig cells occurs in gonadotrophin-deficient mice

Baker, P.J., Johnston, H., Abel, M., Charlton, H.M. and O'Shaughnessy, P.J. (2003) Differentiation of adult-type Leydig cells occurs in gonadotrophin-deficient mice. Reproductive Biology and Endocrinology, 1(4),

[img]
Preview
Text
1477-7827-1-4.pdf

1MB

Publisher's URL: http://www.RBEj.com/content/1/1/4

Abstract

During mammalian testis development distinct generations of fetal and adult Leydig cells arise. Luteinising hormone (LH) is required for normal adult Leydig cell function and for the establishment of normal adult Leydig cell number but its role in the process of adult Leydig cell differentiation has remained uncertain. In this study we have examined adult Leydig cell differentiation in gonadotrophin-releasing hormone (GnRH)-null mice which are deficient in circulating gonadotrophins. Adult Leydig cell differentiation was assessed by measuring expression of mRNA species encoding four specific markers of adult Leydig cell differentiation in the mouse. Each of these markers (3β-hydroxysteroid dehydrogenase type VI (3βHSD VI), 17β-hydroxysteroid dehydrogenase type III (17βHSD III), prostaglandin D (PGD)-synthetase and oestrogen sulphotransferase (EST)) is expressed only in the adult Leydig cell lineage in the normal adult animal. Real-time PCR studies showed that all four markers are expressed in adult GnRH-null mice. Localisation of 3βHSD VI and PGD-synthetase expression by in situ hybridisation confirmed that these genes are expressed in the interstitial tissue of the GnRH-null mouse. Treatment of animals with human chorionic gonadotrophin increased expression of 3βHSD VI and 17βHSD III within 12 hours further indicating that differentiated, but unstimulated cells already exist in the GnRH-null mouse. Thus, while previous studies have shown that LH is required for adult Leydig cell proliferation and activity, results from the present study show that adult Leydig cell differentiation will take place in animals deficient in LH.

Item Type:Articles
Keywords:testis GnRH-null; LH; adult Leydig cells
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:O'Shaughnessy, Professor Peter and Baker, Dr Paul
Authors: Baker, P.J., Johnston, H., Abel, M., Charlton, H.M., and O'Shaughnessy, P.J.
Subjects:R Medicine > RC Internal medicine
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Reproductive Biology and Endocrinology
Publisher:Biomed Central
ISSN:1477-7827
First Published:First published in Reproductive Biology and Endocrinology 1(4).
Publisher Policy:Reproduced with the permission of the Publisher

University Staff: Request a correction | Enlighten Editors: Update this record