Unexpected differential metabolic responses of Campylobacter jejuni to the abundant presence of glutamate and fucose

van der Hooft, J. J.J., Alghefari, W., Watson, E., Everest, P. , Morton, F. R., Burgess, K. E.V. and Smith, D. G.E. (2018) Unexpected differential metabolic responses of Campylobacter jejuni to the abundant presence of glutamate and fucose. Metabolomics, 14(11), 144. (doi:10.1007/s11306-018-1438-5) (PMID:30830405) (PMCID:PMC6208705)

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Abstract

Introduction: Campylobacter jejuni is the leading cause of foodborne bacterial enteritis in humans, and yet little is known in regard to how genetic diversity and metabolic capabilities among isolates affect their metabolic phenotype and pathogenicity. Objectives: For instance, the C. jejuni 11168 strain can utilize both l-fucose and l-glutamate as a carbon source, which provides the strain with a competitive advantage in some environments and in this study we set out to assess the metabolic response of C. jejuni 11168 to the presence of l-fucose and l-glutamate in the growth medium. Methods: To achieve this, untargeted hydrophilic liquid chromatography coupled to mass spectrometry was used to obtain metabolite profiles of supernatant extracts obtained at three different time points up to 24 h. Results: This study identified both the depletion and the production and subsequent release of a multitude of expected and unexpected metabolites during the growth of C. jejuni 11168 under three different conditions. A large set of standards allowed identification of a number of metabolites. Further mass spectrometry fragmentation analysis allowed the additional annotation of substrate-specific metabolites. The results show that C. jejuni 11168 upon l-fucose addition indeed produces degradation products of the fucose pathway. Furthermore, methionine was faster depleted from the medium, consistent with previously-observed methionine auxotrophy. Conclusions: Moreover, a multitude of not previously annotated metabolites in C. jejuni were found to be increased specifically upon l-fucose addition. These metabolites may well play a role in the pathogenicity of this C. jejuni strain.

Item Type:Articles
Keywords:Campylobacter jejuni, metabolomics, HILIC chromatography, mass spectrometry fragmentation, sulphur metabolism.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Burgess, Dr Karl and Everest, Dr Paul and Morton, Mr Fraser
Authors: van der Hooft, J. J.J., Alghefari, W., Watson, E., Everest, P., Morton, F. R., Burgess, K. E.V., and Smith, D. G.E.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Metabolomics
Publisher:Springer
ISSN:1573-3882
ISSN (Online):1573-3890
Published Online:23 October 2018
Copyright Holders:Copyright © 2018 The Authors
First Published:First published in Metabolomics 14(11):144
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
632234Funding SchemesAnna DominiczakWellcome Trust (WELLCOTR)105614/Z/14/ZRI CARDIOVASCULAR & MEDICAL SCIENCES