van der Hooft, J. J.J., Alghefari, W., Watson, E., Everest, P. , Morton, F. R. , Burgess, K. E.V. and Smith, D. G.E. (2018) Unexpected differential metabolic responses of Campylobacter jejuni to the abundant presence of glutamate and fucose. Metabolomics, 14(11), 144. (doi: 10.1007/s11306-018-1438-5) (PMID:30830405) (PMCID:PMC6208705)
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Abstract
Introduction: Campylobacter jejuni is the leading cause of foodborne bacterial enteritis in humans, and yet little is known in regard to how genetic diversity and metabolic capabilities among isolates affect their metabolic phenotype and pathogenicity. Objectives: For instance, the C. jejuni 11168 strain can utilize both l-fucose and l-glutamate as a carbon source, which provides the strain with a competitive advantage in some environments and in this study we set out to assess the metabolic response of C. jejuni 11168 to the presence of l-fucose and l-glutamate in the growth medium. Methods: To achieve this, untargeted hydrophilic liquid chromatography coupled to mass spectrometry was used to obtain metabolite profiles of supernatant extracts obtained at three different time points up to 24 h. Results: This study identified both the depletion and the production and subsequent release of a multitude of expected and unexpected metabolites during the growth of C. jejuni 11168 under three different conditions. A large set of standards allowed identification of a number of metabolites. Further mass spectrometry fragmentation analysis allowed the additional annotation of substrate-specific metabolites. The results show that C. jejuni 11168 upon l-fucose addition indeed produces degradation products of the fucose pathway. Furthermore, methionine was faster depleted from the medium, consistent with previously-observed methionine auxotrophy. Conclusions: Moreover, a multitude of not previously annotated metabolites in C. jejuni were found to be increased specifically upon l-fucose addition. These metabolites may well play a role in the pathogenicity of this C. jejuni strain.
| Item Type: | Articles |
|---|---|
| Keywords: | Campylobacter jejuni, metabolomics, HILIC chromatography, mass spectrometry fragmentation, sulphur metabolism. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Burgess, Dr Karl and Everest, Dr Paul and Morton, Mr Fraser |
| Authors: | van der Hooft, J. J.J., Alghefari, W., Watson, E., Everest, P., Morton, F. R., Burgess, K. E.V., and Smith, D. G.E. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Metabolomics |
| Publisher: | Springer |
| ISSN: | 1573-3882 |
| ISSN (Online): | 1573-3890 |
| Published Online: | 23 October 2018 |
| Copyright Holders: | Copyright © 2018 The Authors |
| First Published: | First published in Metabolomics 14(11):144 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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