Drug resistance mutations in drug-naive HIV type 1 subtype C-infected individuals from rural Malawi

Bansode, V., Drebert, Z. J., Travers, S. A.A., Banda, E., Molesworth, A., Crampin, A. , Ngwira, B., French, N., Glynn, J. R. and McCormack, G. P. (2011) Drug resistance mutations in drug-naive HIV type 1 subtype C-infected individuals from rural Malawi. AIDS Research and Human Retroviruses, 27(4), pp. 439-444. (doi: 10.1089/aid.2010.0203) (PMID:20950147) (PMCID:PMC3068870)

Abstract

In this preliminary study we show that in 2008, 3 years after antiretroviral therapy was introduced into the Karonga District, Malawi, a greater than expected number of drug-naive individuals have been infected with HIV-1 subtype C virus harboring major and minor drug resistance mutations (DRMs). From a sample size of 40 reverse transcriptase (RT) consensus sequences from drug-naive individuals we found five showing NRTI and four showing NNRTI mutations with one individual showing both. From 29 protease consensus sequences, again from drug-naive individuals, we found evidence of minor DRMs in three. Additional major and minor DRMs were found in clonal sequences from a number of individuals that were not present in the original consensus sequences. This clearly illustrates the importance of sequencing multiple HIV-1 variants from individuals to fully assess drug resistance.

• Item Type: Articles
• Additional Information: This material is based upon works supported by Science Foundation Ireland under Grant No. 07/RFP/EEEOBF424 and 08/UR/B1350. The Karonga Prevention Study is funded primarily by the Wellcome Trust, with contributions from LEPRA.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Crampin, Professor Mia
• Authors: Bansode, V., Drebert, Z. J., Travers, S. A.A., Banda, E., Molesworth, A., Crampin, A., Ngwira, B., French, N., Glynn, J. R., and McCormack, G. P.
• College/School: College of Medical Veterinary and Life Sciences > School of Health & Wellbeing > Public Health
• Journal Name: AIDS Research and Human Retroviruses
• Publisher: Mary Ann Liebert
• ISSN: 0889-2229
• ISSN (Online): 1931-8405