HIV type 1 mutational patterns in HIV type 1 subtype C-infected long-term survivors in Karonga District Malawi: further analysis and correction

Seager, I., Leeson, M. D., Crampin, A. C. , Mulawa, D., French, N., Glynn, J. R., Travers, S. A.A. and McCormack, G. P. (2012) HIV type 1 mutational patterns in HIV type 1 subtype C-infected long-term survivors in Karonga District Malawi: further analysis and correction. AIDS Research and Human Retroviruses, 28(3), pp. 308-313. (doi: 10.1089/aid.2011.0168) (PMID:21702696) (PMCID:PMC3292752)

Abstract

Here we present new sequence data from HIV-1 subtype C-infected long-term survivors (LTS) from Karonga District, Malawi. Gag and env sequence data were produced from nine individuals each of whom has been HIV-1 positive for more than 20 years. We show that the three amino acid deletion in gag p17 previously described from these LTS is not real and was a result of an alignment error. We find that the use of dried blood spots for DNA-based studies is limited after storage for 20 years. We also show some unlikely amino acid changes in env C2-V3 in LTS over time and different patterns of genetic divergence among LTS. Although no clear association between mutations and survival could be shown, amino acid changes that are present in more than one LTS may, in the future, be shown to be important.

• Item Type: Articles
• Additional Information: This material is based upon works supported by Science Foundation Ireland under Grant 07/RFP/EEEOBF424 and by an IRCSET EMBARK Scholarship to Ishla Seager (RS/2007/203). The Karonga Prevention Study is funded primarily by the Wellcome Trust, with contributions from LEPRA.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Crampin, Professor Mia
• Authors: Seager, I., Leeson, M. D., Crampin, A. C., Mulawa, D., French, N., Glynn, J. R., Travers, S. A.A., and McCormack, G. P.
• College/School: College of Medical Veterinary and Life Sciences > School of Health & Wellbeing > Public Health
• Journal Name: AIDS Research and Human Retroviruses
• Publisher: Mary Ann Liebert
• ISSN: 0889-2229
• ISSN (Online): 1931-8405
• Published Online: 05 March 2012