Gutierrez-Mecinas, M. , Bell, A. M. , Shepherd, F., Polgár, E., Watanabe, M., Furuta, T. and Todd, A. J. (2019) Expression of cholecystokinin by neurons in mouse spinal dorsal horn. Journal of Comparative Neurology, 527(11), pp. 1857-1871. (doi: 10.1002/cne.24657) (PMID:30734936) (PMCID:PMC6563475)
|
Text
179281.pdf - Published Version Available under License Creative Commons Attribution. 9MB |
Abstract
Excitatory interneurons account for the majority of dorsal horn neurons, and are required for perception of normal and pathological pain. We have identified largely non-overlapping populations in laminae I-III, based on expression of substance P, gastrin-releasing peptide, neurokinin B and neurotensin. Cholecystokinin (CCK) is expressed by many dorsal horn neurons, particularly in the deeper laminae. Here we have used immunocytochemistry and in situ hybridisation to characterise the CCK cells. We show that they account for ~7% of excitatory neurons in laminae I-II, but between a third and a quarter of those in lamina III. They are largely separate from the neurokinin B, neurotensin and gastrin-releasing peptide populations, but show limited overlap with the substance P cells. Laminae II-III neurons with protein kinase Cγ (PKCγ) have been implicated in mechanical allodynia following nerve injury, and we found that around 50% of CCK cells were PKCγ-immunoreactive. Neurotensin is also expressed by PKCγ cells, and among neurons with moderate to high levels of PKCγ, ~85% expressed CCK or neurotensin. A recent transcriptomic study (Häring et al, Nat. Neurosci., 2018) identified mRNA for thyrotropin-releasing hormone (TRH) in a specific subpopulation of CCK neurons, and we show that these account for half of the CCK/PKCγ cells. These findings indicate that CCK cells are distinct from other excitatory interneuron populations that we have defined. They also show that PKCγ cells can be assigned to different classes based on neuropeptide expression, and it will be important to determine the differential contribution of these classes to neuropathic allodynia.
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Bell, Mr Andrew and Beresford-Polgar, Dr Erika and Todd, Professor Andrew and Gutierrez-Mecinas, Dr Maria |
Authors: | Gutierrez-Mecinas, M., Bell, A. M., Shepherd, F., Polgár, E., Watanabe, M., Furuta, T., and Todd, A. J. |
College/School: | College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
Journal Name: | Journal of Comparative Neurology |
Publisher: | Wiley |
ISSN: | 0021-9967 |
ISSN (Online): | 1096-9861 |
Published Online: | 08 February 2019 |
Copyright Holders: | Copyright © 2019 The Authors |
First Published: | First published in Journal of Comparative Neurology 527(11): 1857-1871 |
Publisher Policy: | Reproduced under a Creative Commons License |
University Staff: Request a correction | Enlighten Editors: Update this record