Differential expression of multidrug resistance genes in naïve rat brain

Kwan, P., Sills, G. J., Butler, E., Gant, T. W. and Brodie, M. J. (2003) Differential expression of multidrug resistance genes in naïve rat brain. Neuroscience Letters, 339(1), pp. 33-6. (PMID:12618294)

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Abstract

The multidrug resistance (mdr1) gene family encodes the efflux transporter P-glycoprotein (P-gp) which contributes to the functionality of the blood-brain barrier. We have characterised the regional expression of mdr1 genes in nai;ve rat brain. Adult male Sprague-Dawley rats (n=6) were sacrificed and their brains rapidly removed. Seven distinct anatomical regions were isolated by microdissection and the expression of mdr1a and mdr1b determined by quantitative reverse-transcriptase polymerase chain reaction. The mdr1a isoform was expressed in all brain regions investigated, while mdr1b was expressed to a quantifiable degree in hippocampus alone. These findings reveal a differential expression of mdr1 genes in normal rodent brain tissue and suggest that P-gp may afford a broader protection of the hippocampus than other brain structures.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Sills, Dr Graeme
Authors: Kwan, P., Sills, G. J., Butler, E., Gant, T. W., and Brodie, M. J.
College/School:College of Medical Veterinary and Life Sciences
Journal Name:Neuroscience Letters
Publisher:Elsevier
ISSN:0304-3940
ISSN (Online):1872-7972

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