Determination of gabapentin in plasma by high-performance liquid chromatography.

Forrest, G., Sills, G.J., Leach, J.P. and Brodie, M.J. (1996) Determination of gabapentin in plasma by high-performance liquid chromatography. Journal of Chromatography B, 681(2), pp. 421-425. (PMID:8811458)

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A rapid and simple method for determination of the novel antiepileptic compound gabapentin [1-(aminomethyl)cyclohexaneacetic acid] in plasma is described. Blank human plasma was spiked with gabapentin (1.0-10.0 micrograms/ml) and internal standard [1-(aminomethyl)-cycloheptaneacetic acid; 5.0 micrograms/ml]. Individual samples were treated with 2 M perchloric acid, centrifuged and then derivatised with o-phthalaldehyde-3-mercaptopropionic acid. Separation was achieved on a Beckman Ultrasphere 5 microns reversed-phase column with mobile phase consisting of 0.33 M acetate buffer (pH 3.7; containing 100 mg/l EDTA)-methanol-acetonitrile (40:30:30, v/v). Eluents were monitored by fluorescence spectroscopy with excitation and emission wavelengths of 330 and 440 nm, respectively. The calibration curve for gabapentin in plasma was linear (r = 0.9997) over the concentration range 1.0-10.0 micrograms/ml. Recovery was seen to be > or = 90%. The inter- and intra-assay variations for three different gabapentin concentrations were < or = 10% throughout. The lower limit of quantitation was found to be 0.5 microgram/ml. Chromatography was unaffected by a range of commonly employed antiepileptic drugs or selected amino acids.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Sills, Dr Graeme
Authors: Forrest, G., Sills, G.J., Leach, J.P., and Brodie, M.J.
College/School:College of Medical Veterinary and Life Sciences
Journal Name:Journal of Chromatography B
ISSN (Online):1873-376X

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