Brito, A., Abul-Haija, Y. M. , Soares da Costa, D., Novoa-Carballal, R., Reis, R. L., Ulijn, R., Pires, R. A. and Pashkuleva, I. (2019) Minimalistic supramolecular proteoglycan mimics by co-assembly of aromatic peptide and carbohydrate amphiphiles. Chemical Science, 10, pp. 2385-2390. (doi: 10.1039/C8SC04361B) (PMID:30881666) (PMCID:PMC6385665)
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Abstract
We report on the co-assembly of aromatic carbohydrate and dipeptide amphiphiles under physiological conditions as a strategy to generate minimalistic proteoglycan mimics. The resulting nanofibers present a structural, fluorenylmethoxycarbonyl-diphenylalanine (Fmoc-FF) core and a functional carbohydrate (Fmoc- glucosamine-6-sulfate or –phosphate) shell. The size, degree of bundling and mechanical properties of the assembled structures depend on the chemical nature of the carbohydrate amphiphile used. In cell culture medium, these nanofibers can further organize into supramolecular hydrogels. We demonstrate that, similar to proteoglycans, the assembled gels prolong the stability of growth factors and preserve the viability of cultured cells. Our results demonstrate that this approach can be applied to the design of extracellular matrix (ECM) substitutes for future regenerative therapies.
Item Type: | Articles |
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Additional Information: | We acknowledge the EU’s H2020 and FP7 framework programmes (Forecast 668983; CHEM2NATURE 692333; THE DISCOVERIES CTR 739572; ERC AdG ComplexiTE 321266) and the Portuguese FCT (IF/00032/2013; BD/113794/2015; BPD/85790/2012; M-ERA-NET2/0001/2016. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Abul-Haija, Yousef |
Authors: | Brito, A., Abul-Haija, Y. M., Soares da Costa, D., Novoa-Carballal, R., Reis, R. L., Ulijn, R., Pires, R. A., and Pashkuleva, I. |
College/School: | College of Science and Engineering > School of Chemistry |
Journal Name: | Chemical Science |
Publisher: | Royal Society of Chemistry |
ISSN: | 2041-6520 |
ISSN (Online): | 2041-6539 |
Published Online: | 21 December 2018 |
Copyright Holders: | Copyright © 2019 The Royal Society of Chemistry |
First Published: | First published in Chemical Science 10:2385-2390 |
Publisher Policy: | Reproduced under a Creative Commons License |
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