High-fidelity imaging in brain-wide structural studies using light-sheet microscopy

Müllenbroich, M. C. , Silvestri, L., Di Giovanna, A. P., Mazzamuto, G., Costantini, I., Sacconi, L. and Pavone, F. S. (2018) High-fidelity imaging in brain-wide structural studies using light-sheet microscopy. eNeuro, 5(6), e0124-18.2018. (doi:10.1523/ENEURO.0124-18.2018)

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Light-sheet microscopy (LSM) has proven a useful tool in neuroscience to image whole brains with high frame rates at cellular resolution and, in combination with tissue clearing methods, is often employed to reconstruct the cyto-architecture over the intact mouse brain. Inherently to LSM, however, residual opaque objects, always present to some extent even in extremely well optically cleared samples, cause stripe artifacts, which, in the best case, severely affect image homogeneity and, in the worst case, completely obscure features of interest. Here, demonstrating two example applications in intact optically cleared mouse brains, we report how Bessel beams reduce streaking artifacts and produce high-fidelity structural data for the brain-wide morphology of neuronal and vascular networks. We found that a third of the imaged volume of the brain was affected by strong striated image intensity inhomogeneity and, furthermore, a significant amount of information content lost with Gaussian illumination was accessible when interrogated with Bessel beams. In conclusion, Bessel beams produce high-fidelity structural data of improved image homogeneity and might significantly relax demands placed on the automated tools to count, trace, or segment fluorescent features of interest.

Item Type:Articles
Additional Information:This project was supported by European Union’s H2020 Research and Innovation Programme Grants 720270 (Human Brain Project) and 654148 (Laserlab-Europe) and by the European Union’s Programme H2020 EXCELLENT SCIENCE - European Research Council (ERC) Grant 692943 (BrainBIT). The project has also been supported by the Italian Ministry for Education, University, and Research in the framework of the Flagship Project NanoMAX and of Eurobioimaging Italian Nodes (ESFRI research infrastructure), and by “Ente Cassa di Risparmio di Firenze” (private foundation).
Glasgow Author(s) Enlighten ID:Muellenbroich, Dr Caroline
Authors: Müllenbroich, M. C., Silvestri, L., Di Giovanna, A. P., Mazzamuto, G., Costantini, I., Sacconi, L., and Pavone, F. S.
College/School:College of Science and Engineering > School of Physics and Astronomy
Journal Name:eNeuro
Publisher:Society for Neuroscience
ISSN (Online):2373-2822
Published Online:14 November 2018
Copyright Holders:Copyright © 2018 Müllenbroich et al.
First Published:First published in eNeuro 5(6): e0124-18.2018
Publisher Policy:Reproduced under a Creative Commons License

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