A role for NANOG in G1 to S transition in human embryonic stem cells through direct binding of CDK6 and CDC25A

Zhang, X. et al. (2009) A role for NANOG in G1 to S transition in human embryonic stem cells through direct binding of CDK6 and CDC25A. Journal of Cell Biology, 184(1), pp. 67-82. (doi:10.1083/jcb.200801009)

Full text not currently available from Enlighten.

Abstract

In this study, we show that NANOG, a master transcription factor, regulates S-phase entry in human embryonic stem cells (hESCs) via transcriptional regulation of cell cycle regulatory components. Chromatin immuno-precipitation combined with reporter-based transfection assays show that the C-terminal region of NANOG binds to the regulatory regions of CDK6 and CDC25A genes under normal physiological conditions. Decreased CDK6 and CDC25A expression in hESCs suggest that both CDK6 and CDC25A are involved in S-phase regulation. The effects of NANOG overexpression on S-phase regulation are mitigated by the down-regulation of CDK6 or CDC25A alone. Overexpression of CDK6 or CDC25A alone can rescue the impact of NANOG down-regulation on S-phase entry, suggesting that CDK6 and CDC25A are downstream cell cycle effectors of NANOG during the G1 to S transition.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hoare, Miss Stacey and Keith, Professor Nicol
Authors: Zhang, X., Neganova, I., Przyborski, S., Yang, C.B., Cooke, M., Atkinson, S.P., Anyfantis, G., Fenyk, S., Keith, N., Hoare, S.F., Hughes, O., Strachan, T., Stojkovic, M., Hinds, P.W., Armstrong, L., and Lako, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Journal of Cell Biology
Journal Abbr.:J. Cell Biol.
ISSN:0021-9525
ISSN (Online):1540-8140
Published Online:12 January 2009

University Staff: Request a correction | Enlighten Editors: Update this record